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Structural studies on factor assisted protein folding and targeting

因子辅助蛋白质折叠和靶向的结构研究

基本信息

批准号：
5442577
负责人：
Professorin Paola Fucini, Ph.D.
金额：
--
依托单位：
IKERBASQUE
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2005
资助国家：
德国
起止时间：
2004-12-31 至 2010-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/5442577?language=en
关键词：
Structural studies factor assisted protein

项目摘要

In every organism, translation of the genetic code into functional proteins is performed on the ribosome, a macromolecular machine of more than 2.3 MDa. Since ribosomes play a central cole in cell viability, their activity is intensely regulated by a host of ancillary factors and represent a major target of natural antibiotics. The high-resolution structures of the small (30S) and large (50S) ribosome subunit, in the past few years have revolutionized our understanding of ribosome structure and function. In our group we are in the unique position of being able to prepare four different types of ribosome crystals: Thermus thermophilus 30S and 50S subunits, Deinococcus radiodurans and T. thermophilus 70S ribosome. The aim of this proposal is to utilise the ribosomes and ribosome crystals produced in our laboratory as a platform for the preparation and study of novel ribosomal complexes. Our goals include obtaining high-resolution structures of the ribosome in various functional states: (i) both pre- and post-translocation, (ii) pre-peptide bond formation, as well as (iii) in complex with various translational and regulatory factors. We predict that the results that will be obtained will increase our comprehension of the translational process and will be of potential use for the rational development of new antibiotics.

在每一种生物体中，遗传密码到功能蛋白质的翻译是在核糖体上进行的，核糖体是一种超过2.3 MDa的大分子机器。由于核糖体在细胞活力中起着中心科尔，它们的活性受到许多辅助因子的强烈调节，并且是天然抗生素的主要靶标。在过去的几年里，核糖体小（30S）和大（50S）亚基的高分辨率结构彻底改变了我们对核糖体结构和功能的理解。在我们的小组中，我们处于能够制备四种不同类型的核糖体晶体的独特地位：嗜热栖热菌30S和50S亚基，耐辐射异常球菌和T。嗜热菌70S核糖体该建议的目的是利用我们实验室生产的核糖体和核糖体晶体作为制备和研究新型核糖体复合物的平台。我们的目标包括获得各种功能状态下核糖体的高分辨率结构：（i）易位前和易位后，（ii）前肽键形成，以及（iii）与各种翻译和调节因子的复合物。我们预测，将获得的结果将增加我们的翻译过程的理解，并将是新的抗生素的合理开发的潜在用途。