血小板内在性ＶＥＧＦ－Ａを分子標的とする新規大腸癌治療の臨床及び分子生物学的研究

以血小板固有VEGF-A为分子靶点的新型结直肠癌治疗的临床和分子生物学研究

• 批准号: 16F16420
• 负责人: 井本 浩
• 金额: $ 0.7万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2016
• 资助国家: 日本
• 起止时间: 2016-11-07 至 2018-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16F16420/
• 关键词: Platelet; VEGF-A; Bevacizumab; Pegapyinib; Sunitinib; Colorectal Cancer; Angiogenesis; Pegaptinib

As proposed in our proposal, we have examined two agents in ex vivo and in vitro models (Bevacizumab and Pegaptinib), and only Bevacizumab in human study. We recruited patients diagnosed with advanced colorectal cancer, and isolated the platelets at different interval.We observed a dismal effect of bevacizumab on the concentrations of intra-platelet VEGF-A. The human study results correspond with our ex vivo and in vitro experiments. Compared to serum and plasma, the intra-platelet VEGF-A concentrations remained nearly unchanged. We have also monitored the kinetics of different isoforms of VEGF-A in platelets obtained from the patients before and after Bevacizumab administration. To increase the number in human study, we have recently modified the protocol of patient recruitment.

正如我们的提案中所提出的，我们已经在离体和体外模型中检查了两种药物（贝伐珠单抗和Pegaptinib），并且在人体研究中仅检查了贝伐珠单抗。我们招募了确诊为晚期结直肠癌的患者，并在不同的时间间隔分离血小板，我们观察到贝伐单抗对血小板内VEGF-A浓度的不良影响。人体研究结果与我们的离体和体外实验一致。与血清和血浆相比，血小板内VEGF-A浓度几乎保持不变。我们还监测了贝伐单抗给药前后患者血小板中VEGF-A不同亚型的动力学。为了增加人体研究的数量，我们最近修改了患者招募的方案。

项目成果

Intra-Platelet Serotonin After Partial Hepatectomy: A Candidate Predictor of HCC recurrence

部分肝切除术后血小板内血清素：HCC 复发的候选预测因子

• 发表时间: 2017
• 作者: Bibek Aryal;Toshiaki Shimizu;Jun Kadono;Akira Furoi;Teruo Komokata;Munekazu Yamakuchi ,Teruto Hashiguchi ,Yutaka Imoto
• 通讯作者: Munekazu Yamakuchi ,Teruto Hashiguchi ,Yutaka Imoto

Conquering the deadly stroke: Perspective on a surgeons odyssey

战胜致命的中风：外科医生的冒险之旅的视角

• 发表时间: 2017
• 期刊: Annals of medicine and surgery
• 影响因子: 1.7
• 作者: Aryal B;Shimizu T;Kadono J;Furoi A;Komokata T;Kitazono I;Koriyama C;Yamakuchi M;Hashiguchi T;Imoto Y.;Bibek Aryal
• 通讯作者: Bibek Aryal

Qualitative and Quantitative Platelet Crisis In Cancer (Hepatocellular) Recurrence

癌症（肝细胞）复发中的定性和定量血小板危机

• 发表时间: 2018
• 作者: Aryal B;Shimizu T;Kadono J;Furoi A;Komokata T;Kitazono I;Koriyama C;Yamakuchi M;Hashiguchi T;Imoto Y.;Bibek Aryal;Bibek Aryal;Bibek Aryal
• 通讯作者: Bibek Aryal

A 2-stage surgical and endovascular treatment of rare multiple anuerysms of pancreatic arteries

罕见的多发性胰动脉瘤的两阶段手术和血管内治疗

• 发表时间: 2017
• 期刊: Annals of vascular surgery
• 影响因子: 1.5
• 作者: Aryal B;Shimizu T;Kadono J;Furoi A;Komokata T;Kitazono I;Koriyama C;Yamakuchi M;Hashiguchi T;Imoto Y.;Bibek Aryal;Bibek Aryal
• 通讯作者: Bibek Aryal

Perioperative Serum PDGF-BB as a Predictor of Post-resection HCC Recurrence

围手术期血清 PDGF-BB 作为切除后 HCC 复发的预测因子

• 发表时间: 2018
• 作者: Aryal B;Shimizu T;Kadono J;Furoi A;Komokata T;Kitazono I;Koriyama C;Yamakuchi M;Hashiguchi T;Imoto Y.;Bibek Aryal;Bibek Aryal;Bibek Aryal;Bibek Aryal
• 通讯作者: Bibek Aryal