インフルエンザウイルスのゲノムパッケージング機構の解明

在这个项目中，我们使用了一种结合了细胞去顶方法、冷冻蚀刻电子显微镜和活原子力显微镜的纳米方法来解决病毒粒子形成和细胞机制参与甲型流感病毒(IAV)复制的机制。通过使用免疫标记来鉴定先前实验中发现的各种细胞蛋白，我们验证了几个关键宿主因子(S)以及它们如何与病毒组件相互作用，以促进病毒基因组的组装和萌发(手稿正在准备中)。我还参与了另一个项目，旨在开发活体成像分析方法，以研究流感病毒感染小鼠肺部的疾病进展。我们揭示了流感病毒感染诱导的肺血管内中性粒细胞的早期和短暂招募(Ueki，Wang等人，PNAS，2018)。

In this project, we employed a nanoscopic approach that combines a cell unroofing method, freeze-etching electron microscopy and live atomic force microscopy to resolve the mechanism behind virion formation and the involvement of cellular machineries in influenza A virus (IAV) replication. By using immuno-labeling to identify various cellular proteins found in the previous experiments, we verified few key host factor(s) and how they interact with viral components to facilitate the viral genome assembly and budding (a manuscript in preparation).I also worked on the another project which was aimed at developing intravital imaging assays to study disease progression in the lung of influenza virus-infected mice. We revealed an early and transient recruitment of pulmonary intravascular neutrophils induced by influenza virus infection (Ueki, Wang et al., PNAS, 2018).