Role of symbiotic assemblages in coral host survival: translocation from mesophotic to shallow reef

共生组合在珊瑚宿主生存中的作用：从中光到浅礁的易位

• 批准号: 16F16780
• 负责人: 波利井 佐紀
• 金额: $ 1.41万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2016
• 资助国家: 日本
• 起止时间: 2016-11-07 至 2019-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16F16780/
• 关键词: coral; endosymbionts; exosymbionts; diversity; mesophotic; mesophotic corals; translocation

In FY2018, we finished the transplantation experiment with the final samplings of the corals and exosymbionts and the molecular analyses of both endo- and exosymbionts. We also performed nutriment and stable isotopes measurements from water sampled at the original and two transplant depths and the corals in collaboration with Dr. Yamazaki and Dr. Watanabe at Hokkaido University. In addition, Dr. Rouze, the JSPS fellow, visited Dr. Naruse, an expert in crustacean taxonomy in Iriomote Station of our University to work together on the taxonomy of a new exosymbiont species. The preliminary data obtained in this project were presented at the Gordon Conference on Mesophotic Coral Ecosystems in USA in June 2018.The massive amount of data obtained by high throughput sequencing of the endosymbionts (Symbiodiniaceae) is still being analysed but the first results suggest a relative qualitative stability over depth contrasting with the quantitative results obtained by qPCR. In parallel, the stable isotopes analyses evidenced the nutritional plasticity of the coral Seriatopora hystrix.Manuscripts reporting these finding are currently being finalized, while a manuscript on the early onset of the symbiosis between Acropora corals and Tetralia crabs has been published in April 2019.

2018财年，我们完成了珊瑚和外部共生体的最终采样和内外共生体的分子分析的移植实验。我们还与北海道大学的山崎博士和渡边博士合作，对原始和两个移植深度的水样以及珊瑚进行了营养和稳定同位素测量。此外，JSPS研究员Rouze博士拜访了我校Iriomote站甲壳类分类专家Naruse博士，共同研究一种新的外部共生体物种的分类。该项目获得的初步数据已于2018年6月在美国举行的戈登中栖珊瑚生态系统会议上公布。通过高通量测序获得的大量内共生菌(共生菌科)数据仍在分析中，但第一个结果表明，与qPCR获得的定量结果相比，在深度上具有相对的定性稳定性。同时，稳定同位素分析证明了珊瑚Seriatopora hystrix的营养可塑性。报告这些发现的手稿目前正在定稿，而关于Acropora珊瑚和Tetralia蟹之间早期共生的手稿已于2019年4月出版。

项目成果

Symbiotic communities associated to corals from mesophotic vs. shallower coral ecosystems in Okinawa (Japan): implications for the coral-guilds depth adaptation

冲绳（日本）中光珊瑚与浅层珊瑚生态系统中珊瑚的共生群落：对珊瑚行会深度适应的影响

• 发表时间: 2018
• 作者: Rouze H;Sinniger F;Harii S
• 通讯作者: Harii S