Structural mechanism of the activation of RIG-I by viral RNA (A13)

病毒RNA激活RIG-I的结构机制（A13）

• 批准号: 5447624
• 金额: --
• 依托单位: Genzentrum - Laboratorium für molekulare Biologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5447624?language=en
• 关键词: Structural; mechanism; activation; RIG; viral

The ATPase RIG-I senses the presence of viral RNA in the cytoplasm and initiates a signal transduction cascade that leads to stimulation of the innate immune response. We have obtained first structural insights into recognition of viral RNA by RIG-I. In this proposal we want to derive a complete structural mechanism of activation of RIG-I by viral RNA. In collaboration we address by structural, biochemical and in vivo functional approaches, how RIG-I binds viral RNA and how RNA and ATP binding induce a signaling conformation in RIG-I that leads to stimulation of the innate immune response.

ATP酶RIG-I感知细胞质中病毒RNA的存在，并启动信号转导级联，导致刺激先天免疫应答。 我们已经获得了RIG-I识别病毒RNA的第一个结构见解。在这个提议中，我们希望得到一个完整的结构机制激活RIG-I的病毒RNA。 在合作中，我们解决了结构，生物化学和体内功能的方法，RIG-I如何结合病毒RNA和RNA和ATP结合如何诱导RIG-I的信号传导构象，导致刺激先天免疫反应。