Field-assisted assembly of the DNA networks for electronics and biologydcal applications

用于电子和生物应用的 DNA 网络现场辅助组装

• 批准号: 17F17065
• 负责人: 松本 卓也
• 金额: $ 1.47万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2017
• 资助国家: 日本
• 起止时间: 2017-04-26 至 2019-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17F17065/
• 关键词: nanopipette; DNA; patterning; surface modification; DNA electronics; field-assisteddepo.; nanofluidics; liquid metals

In the final year of the JSPS fellowship (2018-2019), we established new tools and methodologies to address technical difficulties encountered during our research. Most significantly, we succeeded in optimizing buffer conditions for the deposition of ligated λ-DNA (~100 µm in length) on APTES-coated glass slides using the nanopipette with a counter electrode. Imaging of the patterned lines was done in a fluorescence microscope.We successfully implemented new strategies to overcome significant resistant to flow of water and liquid metals as channel cross section decreases in diameter, from ~500 um to ~100 nm:1. A new pipette holder was constructed, enabling simultaneous application of high pressure (up to ~200 Atm), heating (up to ~500 C) and high voltage (up to 10 kV) to pipette. Using the new holder, we were able to extrude GaIn alloy through the nanopipette channel.2. Protocol for coating of the inside of glass capillaries with gold using electroless plating was established, with successful reproducible results. Gold coated nanopipettes will be used for various applications.

在JSPS奖学金的最后一年(2018-2019年)，我们建立了新的工具和方法，以解决我们在研究过程中遇到的技术困难。最重要的是，我们成功地优化了使用带有对电极的纳米电极在APTE涂层玻片上沉积连接的λ-DNA(长度约100微米)的缓冲条件。我们成功地实施了新的策略，以克服随着通道横截面直径从~500微米减小到~100 nm而对水和液体金属流动的显著阻力：1.构建了一个新的吸管支架，可以同时对吸管施加高压(高达~200 atm)、加热(高达~500℃)和高压(高达10千伏)。使用新的夹持器，我们能够通过纳米管通道挤压增益合金。建立了用化学镀的方法在玻璃毛细管内壁镀金的方法，并获得了成功的重复性结果。镀金纳米管将用于各种应用。

项目成果

ナノピペット技法を用いた固体表面上のDNAパターニング

使用纳米移液器技术在固体表面上绘制 DNA 图案

• 发表时间: 2018
• 作者: Gideon Issac Livshits;松本卓也
• 通讯作者: 松本卓也

松本研究室

松本实验室