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Study of Rapid Detection Technology for Oil Quality and Toxicological Evaluation of Frying Oil

煎炸油油质快速检测技术及毒理学评价研究

基本信息

批准号：
17F17751
负责人：
惠淑萍
金额：
$ 1.41万
依托单位：
Hokkaido University (2018-2019)Akita Prefectural University (2017)
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2017
资助国家：
日本
起止时间：
2017-11-10 至 2020-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17F17751/
关键词：
Hydroperoxide,cell toxicity,fatty acid triacylglycerol TGOOH hydroperoxides Frying oil quality evaluation e-nose polar compounds toxicity in vitro

项目摘要

It was established that an effective and rapid detection method based on the odor fingerprint of frying oil for a real-time detection and monitoring of frying oil quality. The potential toxicities of frying oils' polar substances and oxidative products (hydroperoxides) to cells in vivo were investigated. The major primary oxidative products of the linoleic acid, 9 and 13-Hydroxyoctadecadienoic acid (9 and 13-HPODE), were synthesized using soybean lipoxygenase I-B. The 9 and 13-HPODE were also subjected to HepG 2 cells to evaluate its possible adverse effects (cell viability, changes in the enzyme expression, lipidomic analysis) . The research results of 2019 are summarized as follows:1.The synthesized hydroperoxides have been identified by the Mass spectroscopy and NMR. Results showed that the purity of the synthesized 13-HPODE was around 95% and the yield rate was 70% above which implies the method is successful and was feasible for preparing 9 and 13-HPODE in a regular laboratory.2.In the cells’ experiments, there were two parallel experiments carried out: one is for the PAHs and another is for the HPODEs using target cell line- HepG 2 cells. Results showed that the mRNA expression of GSTO1, SOD2, and HO-1 have increased when the cells were treated with high HPODEs concentration (100 uM), while the GPX mRNA expression was inhibited after the treatment. Results also indicated that the expression level of CYP1A1 mRNA has been significantly increased after the cells treated with high concentration of HPODEs.

建立了一种有效、快速的基于煎炸油气味指纹图谱的检测方法，实现了煎炸油质量的实时检测与监控。研究了煎炸油极性物质和氧化产物（氢过氧化物）对体内细胞的潜在毒性。利用大豆脂氧合酶I-B合成了亚油酸的主要初级氧化产物9和13-羟基十八烯二烯酸（9和13-HPODE）。9和13-HPODE也被用于HepG 2细胞，以评估其可能的不良反应（细胞活力，酶表达的变化，脂质组学分析）。2019年的研究成果总结如下：合成的氢过氧化物经质谱和核磁共振鉴定。结果表明，合成的13-HPODE纯度在95%左右，产率在70%以上，表明该方法是成功的，在常规实验室制备9和13-HPODE是可行的。在细胞实验中，平行进行了两个实验：一个是PAHs实验，另一个是HPODEs实验，使用目标细胞系HepG - 2细胞。结果表明，高HPODEs浓度（100 uM）处理后，GSTO1、SOD2和HO-1 mRNA表达增加，GPX mRNA表达抑制。结果还表明，高浓度HPODEs处理细胞后，CYP1A1 mRNA的表达水平显著升高。