Study of Rapid Detection Technology for Oil Quality and Toxicological Evaluation of Frying Oil

建立了一种基于煎炸油气味指纹的快速有效检测方法，实现了煎炸油质量的实时检测和监控。研究了煎炸油中极性物质和氧化产物（氢过氧化物）对细胞的潜在毒性。利用大豆脂肪氧合酶I-B合成了亚油酸的主要初级氧化产物9和13-羟基十八碳二烯酸（9和13-HPODE）。9和13-HPODE也经受HepG 2细胞以评估其可能的不利影响（细胞活力、酶表达的变化、脂质组学分析）。2019年的研究成果总结如下：1.通过质谱和核磁共振对合成的氢过氧化物进行了鉴定。结果表明，合成的13-HPODE纯度在95%左右，产率在70%以上，说明该方法是成功的，在常规实验室中制备9和13-HPODE是可行的。2.在细胞实验中，进行了两个平行的实验：一个是多环芳烃的实验，另一个是以靶细胞HepG 2为靶细胞的HPODE的实验。结果表明，高浓度HPODEs（100 μ M）处理后，GSTO 1、SOD 2和HO-1的mRNA表达增加，GPX mRNA表达受到抑制。高浓度HPODEs处理后，细胞CYP 1A 1 mRNA的表达水平显著升高。

It was established that an effective and rapid detection method based on the odor fingerprint of frying oil for a real-time detection and monitoring of frying oil quality. The potential toxicities of frying oils' polar substances and oxidative products (hydroperoxides) to cells in vivo were investigated. The major primary oxidative products of the linoleic acid, 9 and 13-Hydroxyoctadecadienoic acid (9 and 13-HPODE), were synthesized using soybean lipoxygenase I-B. The 9 and 13-HPODE were also subjected to HepG 2 cells to evaluate its possible adverse effects (cell viability, changes in the enzyme expression, lipidomic analysis) . The research results of 2019 are summarized as follows:1.The synthesized hydroperoxides have been identified by the Mass spectroscopy and NMR. Results showed that the purity of the synthesized 13-HPODE was around 95% and the yield rate was 70% above which implies the method is successful and was feasible for preparing 9 and 13-HPODE in a regular laboratory.2.In the cells’ experiments, there were two parallel experiments carried out: one is for the PAHs and another is for the HPODEs using target cell line- HepG 2 cells. Results showed that the mRNA expression of GSTO1, SOD2, and HO-1 have increased when the cells were treated with high HPODEs concentration (100 uM), while the GPX mRNA expression was inhibited after the treatment. Results also indicated that the expression level of CYP1A1 mRNA has been significantly increased after the cells treated with high concentration of HPODEs.