Subcortical processing of visual fear stimuli in the common marmoset

普通狨猴视觉恐惧刺激的皮层下处理

基本信息

批准号：
18F18105
负责人：
伊佐正
金额：
$ 1.47万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2018
资助国家：
日本
起止时间：
2018-04-25 至 2020-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18F18105/
关键词：
marmoset innate fear amygdala superior colliculus pupillometry eye movement

项目摘要

I have finished my JSPS fellow and am grateful that JSPS gave me such a good opportunity with funding to support my research. I have achieved several important milestones regarding my research. First, I have recorded eye movements from head-restrained marmosets while they were viewing fearful and non-fearful pictures. It was clear that they spent more time looking at the fearful pictures. I have also successfully tested several different 3D printed implants on the marmosets. Furthermore, I have trained the marmosets with saccade tasks and I am currently writing a manuscript on the results. As a side product, I have also successfully performed microstimulation in marmoset cortex and identified an important brain area related to saccade. I have finished the data acquisition and will start to write the manuscript soon. I also successfully established method for brain microinjection on marmosets and successfully traced the superior colliculus. This is very important result. As what stated last year, I have also performed double injection, one anterograde in the superior colliculus and one retrograde in the amygdala in the same marmoset to exploring the connection between the two brain areas. I am currently testing several types of AAV virus to have good expression for future optogenetic experiments. With the grant provided by JSPS, I have also participated several local and international conferences to present my work and got feedbacks from other researchers. Overall, I believe I have reached several goals I set before and I would like to continue my research about innate fear.

我已经完成了JSP的研究员，并感谢JSP给我一个很好的机会，可以通过资金来支持我的研究。关于我的研究，我取得了一些重要的里程碑。首先，我在观看恐惧和非恐惧的照片时录制了眼睛架的果饼的眼睛动作。很明显，他们花了更多时间看可怕的照片。我还成功地测试了Marmoset上的几种不同的3D印刷植入物。此外，我已经通过扫视任务培训了Marmosets，目前正在撰写有关结果的手稿。作为侧产品，我还成功地在Marmoset皮层中进行了微刺激，并确定了与扫视有关的重要大脑区域。我已经完成了数据采集，并将很快开始编写手稿。我还成功地建立了对摩尔果的大脑显微注射的方法，并成功地追溯了上丘。这是非常重要的结果。如去年所述，我还进行了双重注射，在上山脉中进行了一只船尾，在同一Marmoset中的杏仁核中进行了1次逆行，以探索两个大脑区域之间的联系。目前，我正在测试几种AAV病毒，以表达良好的表达，用于未来的光遗传实验。根据JSP提供的赠款，我还参加了几次本地和国际会议，以介绍我的作品，并获得了其他研究人员的反馈。总体而言，我相信我已经达到了以前设定的几个目标，并且我想继续对先天恐惧的研究。