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Subcortical processing of visual fear stimuli in the common marmoset

普通狨猴视觉恐惧刺激的皮层下处理

基本信息

批准号：
18F18105
负责人：
伊佐正
金额：
$ 1.47万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2018
资助国家：
日本
起止时间：
2018-04-25 至 2020-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18F18105/
关键词：
marmoset innate fear amygdala superior colliculus pupillometry eye movement

项目摘要

I have finished my JSPS fellow and am grateful that JSPS gave me such a good opportunity with funding to support my research. I have achieved several important milestones regarding my research. First, I have recorded eye movements from head-restrained marmosets while they were viewing fearful and non-fearful pictures. It was clear that they spent more time looking at the fearful pictures. I have also successfully tested several different 3D printed implants on the marmosets. Furthermore, I have trained the marmosets with saccade tasks and I am currently writing a manuscript on the results. As a side product, I have also successfully performed microstimulation in marmoset cortex and identified an important brain area related to saccade. I have finished the data acquisition and will start to write the manuscript soon. I also successfully established method for brain microinjection on marmosets and successfully traced the superior colliculus. This is very important result. As what stated last year, I have also performed double injection, one anterograde in the superior colliculus and one retrograde in the amygdala in the same marmoset to exploring the connection between the two brain areas. I am currently testing several types of AAV virus to have good expression for future optogenetic experiments. With the grant provided by JSPS, I have also participated several local and international conferences to present my work and got feedbacks from other researchers. Overall, I believe I have reached several goals I set before and I would like to continue my research about innate fear.

我已经完成了我的JSPS研究员，并感谢JSPS给了我这样一个很好的机会，资助我的研究。我在我的研究中取得了几个重要的里程碑。首先，我记录了头部受限的绒猴在观看恐惧和非恐惧图片时的眼球运动。很明显，他们花了更多的时间看那些可怕的照片。我还成功地在绒猴身上测试了几种不同的3D打印植入物。此外，我已经用扫视任务训练了绒猴，目前我正在写一份关于结果的手稿。作为一个副产品，我还成功地在绒猴皮层进行了微刺激，并确定了一个与扫视相关的重要大脑区域。我已经完成了数据采集，很快就会开始写稿子。成功建立了绒猴脑显微注射方法，并成功追踪了上级丘。这是非常重要的结果。正如去年所述，我还进行了双重注射，一次在同一只绒猴的上级丘顺行注射，一次在杏仁核逆行注射，以探索两个大脑区域之间的联系。我目前正在测试几种类型的AAV病毒，以便为未来的光遗传学实验提供良好的表达。在JSPS的资助下，我还参加了几次本地和国际会议，介绍我的工作，并得到了其他研究人员的反馈。总的来说，我相信我已经达到了我之前设定的几个目标，我想继续我对先天恐惧的研究。