コモンマーモセットの前頭葉ー頭頂葉系の障害が半側空間無視を誘発する神経機構

去年的主要重点是建立绒猴眼球跟踪研究的实验室。我们开发了一种双重固定绒猴头部的方法：由可注塑塑料制成的挤压头盔从两侧挤压头部，并通过手术植入头柱。最初，主要关注自由观看实验，即挤压头部的绒猴观察自然刺激。在一个试点项目中，我们测试了来自国家放射科学研究所的5只对照和4只聚IC绒猴，显示出一些类似于人类精神分裂症的行为异常。目前，获得的数据正在分析中，我们希望根据结果发表文章。最后，重点已经转移到注视训练上，这可以更精确地校准凝视数据和行为条件。目前已有4只绒猴使用挤头器进行训练，1只绒猴已植入头柱，他的训练也已经开始。已经编写了30多个不同的软件脚本来向绒猴呈现各种视觉刺激。此外，还编写了用于凝视校准和凝视数据在线可视化的独立计算机程序。两只绒猴已经学会了注视中央呈现的刺激。一旦足够多的绒猴接受了注视和眼跳任务的训练，生理和光遗传操作就会紧随其后。对绒猴动眼神经通路的解剖学研究也在进行中，结果将在日本神经科学学会第40届年会上公布。

The main focus of the last year was on building up the laboratory for eye tracking research in marmosets. We developed a dual way to fix the marmoset's head: the squeeze helmet made from moldable plastic pressing the head from two sides and the surgically implantable head-post.Initially, the main focus has been on free-viewing experiments, where the head-squeezed marmosets observed naturalistic stimuli. In a pilot project, we tested 5 controls and 4 poly IC marmosets from the National Institute of Radiological Sciences showing some behavioural abnormalities similar to human schizophrenia. Currently, the obtained data is being analysed and we hope to produce a publication from the results.Lately, the focus has shifted to the fixation training, which allows a more precise calibration of the gaze data and conditioning of the behaviour. 4 marmosets are trained using the head-squeeze now and 1 marmoset has been implanted with the head-post and his training has also begun. More than 30 different software scripts have been written to present marmosets with various visual stimuli. Also stand-alone computer programs for gaze calibration and online visualisation of the gaze data has been written. 2 marmosets have already learned to fixate to the centrally presented stimuli. Physiological and optogenetic manipulations will follow as soon as enough marmosets have been trained in fixation and saccade tasks. Anatomical research of the oculomotor pathways in the marmosets has also been ongoing and results will be presented during the 40th annual meeting of Japan Neuroscience Society.