权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

量的形質遺伝子座の集積による形質発現の安定性－トマトの早期開花性を例に－

通过数量性状位点积累实现性状表达的稳定性 - 以番茄早花为例 -

基本信息

批准号：
18J10247
负责人：
中野玄
金额：
$ 0.96万
依托单位：
The University of Tokyo (2019)Tokyo University of Agriculture (2018)
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2018
资助国家：
日本
起止时间：
2018-04-25 至 2020-03-31
项目状态：
已结题

项目摘要

昨年度までの研究活動によって育成した，第1染色体と第4染色体のそれぞれの早期開花性QTL領域が野生種ホモ型になっている2つの系統（NIL-1とNIL-4）の雑種後代について栽培試験を行い，QTLの効果を評価した．後代の育成の際には，交配親の雌雄の違いによる効果を調査するために，花粉親と胚珠親をそれぞれ入れ替えて交配を行った（NIL1+4，NIL4+1）．トマト栽培種，近縁野生種，NIL-1，NIL-4，NIL1+4，NIL4+1を温室内で栽培し，播種から開花までの日数（到花日数）を子葉展開，蕾出現，開花までのそれぞれの日数に分割して調査した．その結果，栽培種よりもNILと雑種後代の方で到花日数が短いことを確認したが，NILと雑種後代の間では差が認められなかった．到花日数を分割した形質について，子葉展開日数では，NIL-1が花粉親であるNIL1+4が栽培種やNILよりも有意に短くなったことから当該QTL領域には，COTを制御するエピスタシスQTLが存在する可能性が考えられた．しかし，QTL効果の遺伝様式を明らかにするためにはさらに詳細な実験を行う必要がある．本研究は，比較的大きな効果をもつQTLは環境に関わらず安定的に発現することが先行研究で明らかにになっていることから，QTLの効果を集積させることで栽培環境に関わらず農業上有利な形質を発現させることを期待した研究であったが，QTLを集積させることが必ずしも相加効果を示すわけではないという先行研究を確かめる結果となった．

In the past year, the research activities were carried out in breeding, early flowering QTL fields on chromosome 1 and chromosome 4 were selected, and wild type QTL systems (NIL-1 and NIL-4) were selected in breeding, and QTL fruit was evaluated. During breeding, mating parents and female parents were investigated for their mating behavior (NIL 1 + 4, NIL 4 + 1), pollen parents and ovule parents were investigated for their mating behavior (NIL1+4, NIL4+1). Cultivated species, near-wild species, NIL-1, NIL-4, NIL1+4, NIL4+1 are cultivated in greenhouse, the number of days from sowing to flowering (days to flowering), cotyledon development, bud emergence, and the number of days from flowering to flowering. The result of cultivation is that the number of days to flower of the seed cross is short and the difference between the seed cross and the seed cross is short and confirmed. The number of days to flower is divided into two parts, the number of days to cotyledon development is divided into two parts, the number of days to flower is divided into two parts, the number of days to cotyledon development is divided into two parts, the number of days to flower is divided into two parts, the number of days to cotyledon development is divided into two parts, the number of days to flower is divided into two parts, the number of days to flower is divided into two parts, the number of days to cotyledon development is divided into two parts, the number of days to flower is divided into two parts, the number of days to flower is divided into two parts, and the number of days to cotyledon development is divided into two parts. The QTL results are expressed in detail. In this study, we compared the results of QTL aggregation and QTL accumulation in relation to environment and stability.