Study on metabolism/physiological functions of retinoic acid in metamorphic larvae of the Pleuronectiformes

侧鲛目变态幼虫视黄酸的代谢/生理功能研究

• 批准号: 18J20627
• 负责人: 陳 其然
• 金额: $ 1.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-04-25 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18J20627/
• 关键词: RNA-Seq; Retinoic acid; cyp26; Plasmid delivery; Electroporation; Japanese flounder; bco1; retinoic acid; metabolism; in situ hybridization; metamorphosis; retinoic acid signaling

1. RT-PCR confirmed bco1l was down-regulated by RA treatment in the intestine. Members of cyp26 and dhrs3 were up-regulated in both sides of skin, vertebra and gills. The data suggest malformations caused by RA exposure is systemically related to this mechanism.2. The success of transferring plasmid to flounder tissues and bamboo sole ovary eggs provides the basis for technique using CRISPR/Cas9 system in flatfish, which distribute to developmental biology in asymmetry study.3. Genes encoding retinoic acid receptors expressed ubiquitously in G-stage flounders. The expression of rars in both sides of skin and vertebra suggest the skins and vertebra are responsive to RA induction. Bone and skin malformation caused by RA induction at G-stage is related to the expression of these rars.

1.逆转录聚合酶链式反应证实维甲酸处理后bco1基因在肠道中表达下调。Cyp26和dhrs3基因在两侧皮肤、脊椎和鳃中表达上调。这些数据表明，RA暴露引起的畸形与这一机制有系统的关系。本研究成功地将CRISPR/Cas9系统转移到比目鱼组织和竹子房卵中，为CRISPR/Cas9系统在发育生物学不对称性研究中的应用奠定了技术基础。编码维甲酸受体的基因在G期牙鲨中普遍表达。RARs在皮肤和脊椎两侧的表达提示皮肤和脊椎对RA诱导有反应。RA诱导的G期骨骼和皮肤畸形与这些RAR的表达有关。

项目成果

RNA-Seq screening of up- and down-regulated genes by retinoic acid in metamorphosing flounder larvae

RNA-Seq 筛选变态牙鲆幼虫中视黄酸上调和下调基因

• 发表时间: 2019
• 作者: Qiran Chen;Hayato Yokoi;Tohru Suzuki
• 通讯作者: Tohru Suzuki

Retinoic acid is the key regulator of asymmetric pigmentation in the Japanese flounder larvae

视黄酸是日本比目鱼幼虫不对称色素沉着的关键调节剂

• 发表时间: 2021
• 作者: Qiran Chen;Kota Sato;Hayato Yokoi;Tohru Suzuki
• 通讯作者: Tohru Suzuki

Critical role of RA signaling during the asymmetric pigmentation of metamorphosing Japanese flounder

RA信号在日本比目鱼变态不对称色素沉着过程中的关键作用

• 发表时间: 2020
• 作者: Qiran Chen;Kota Sato;Hayato Yokoi;Tohru Suzuki
• 通讯作者: Tohru Suzuki

Retinoic acid plays critical role for flounder asymmetrical pigmentation

视黄酸对比目鱼不对称色素沉着起着关键作用

• 发表时间: 2020
• 作者: Qiran Chen;Hayato Yokoi;Tohru Suzuki
• 通讯作者: Tohru Suzuki