Study on metabolism/physiological functions of retinoic acid in metamorphic larvae of the Pleuronectiformes

1. RT-PCR证实，RA治疗后肠内bcol1下调。cyp26和dhrs3成员在皮肤、脊椎和鳃两侧均上调。数据表明，类风湿性关节炎暴露引起的畸形与这一机制有系统关系。质粒在比目鱼组织和竹比目鱼子房卵上的成功转移，为CRISPR/Cas9系统在比目鱼中的应用提供了技术基础，并将其推广到发育生物学的不对称研究中。编码维甲酸受体的基因在g期牙鲆中普遍表达。rars在皮肤和椎体两侧的表达表明皮肤和椎体对RA诱导有反应。g期RA诱导的骨和皮肤畸形与这些rars的表达有关。

1. RT-PCR confirmed bco1l was down-regulated by RA treatment in the intestine. Members of cyp26 and dhrs3 were up-regulated in both sides of skin, vertebra and gills. The data suggest malformations caused by RA exposure is systemically related to this mechanism.2. The success of transferring plasmid to flounder tissues and bamboo sole ovary eggs provides the basis for technique using CRISPR/Cas9 system in flatfish, which distribute to developmental biology in asymmetry study.3. Genes encoding retinoic acid receptors expressed ubiquitously in G-stage flounders. The expression of rars in both sides of skin and vertebra suggest the skins and vertebra are responsive to RA induction. Bone and skin malformation caused by RA induction at G-stage is related to the expression of these rars.