BAT thermogenesis-triggered organ cross-talk through the exosomal microRNA

BAT 生热作用通过外泌体 microRNA 触发器官串扰

• 批准号: 18J21697
• 负责人: BARIUAN JUSSIAEA VALENTE
• 金额: $ 1.41万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-04-25 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18J21697/
• 关键词: microRNA-122; skeletal muscle; C2C12 cells; interleukin-6; time-response; myostatin; brown adipose tissue; uncoupling protein 1; cold exposure; microRNA; microRNA 122 (miR-122); Uncoupling protein 1; BAT activation; nonessential fatty acids /

To understand the effect of batokines: interleukin-6 (IL-6)and myostatin (GDF 8), on microRNA 122 (miR-122) expression in myocytes, C2C12 myoblasts differentiated for 14 to 16 days and showing myotube formation were treated with two different concentrations of each of the mentioned batokines for 6 hours. After treatment, the microRNA of the cells were harvested and measured. 25ng of IL-6 significantly increased C2C12 miR-122 expression (%) unlike the higher 100ng treatment concentration which only increased the C2C12 by % compared to the control group.To determine the potency of IL-6 effect on miR-122 expression in C2C12 cells, I checked the time- and dose-responses. The effects of 5ng, 10ng and 25ng IL-6 doses on C2C12 were not significantly different from each other. In contrast, a significant difference in the time-response to IL-6 treatment was observed. After 2 hours, miR-122 expression was 56% higher than at the start of the treatment. At 4 hours after treatment, a 53% increase in the miR-122 expression was observed whereas after 8 hours, there was a 31% drop in the miR-122 expression in the C2C12 cells. This indicates a significant time-response of myocyte miR-122 expression to IL-6.On the other hand, GDF8 treatment did not produce any significant effects on C2C12 miR-122 expression

为了了解白细胞介素-6 （IL-6）和肌生长抑制素（GDF - 8）对肌细胞中microRNA 122 （miR-122）表达的影响，C2C12成肌细胞分化14至16天，并显示出肌管形成，用两种不同浓度的上述细胞因子分别处理6小时。处理后，收集细胞的microRNA并测量。25ng IL-6显著增加C2C12 miR-122的表达（%），而更高的100ng处理浓度与对照组相比仅增加C2C12 %。为了确定IL-6对C2C12细胞中miR-122表达的影响，我检查了时间和剂量反应。5ng、10ng和25ng IL-6剂量对C2C12的影响无显著性差异。相比之下，观察到对IL-6治疗的时间反应有显著差异。2小时后，miR-122的表达比治疗开始时高56%。在治疗后4小时，观察到miR-122表达增加53%，而在8小时后，C2C12细胞中miR-122表达下降31%。这表明心肌细胞miR-122表达对IL-6有显著的时间响应。另一方面，GDF8处理没有对C2C12 miR-122的表达产生任何显著影响

项目成果

Cold-induced activation of BAT thermogenesis increases circulating miR-122 level possibly through the secretion from muscle

寒冷诱导的 BAT 产热激活可能通过肌肉分泌增加循环 miR-122 水平

• 发表时间: 2019
• 作者: Jussiaea Valente Bariuan;岡松優子;松岡慎也;坪田あゆみ;斉藤昌之;木村和弘;Jussiaea Valente Bariuan
• 通讯作者: Jussiaea Valente Bariuan

Association of circulating exosomal miR-122 levels with BAT activity in healthy humans

健康人循环外泌体 miR-122 水平与 BAT 活性的关联

• DOI: 10.1038/s41598-019-49754-1
• 发表时间: 2019
• 期刊: Scientific Reports
• 影响因子: 4.6
• 作者: Okamatsu-Ogura Yuko;Matsushita Mami;Bariuan Jussiaea Valente;Nagaya Kazuki;Tsubota Ayumi;Saito Masayuki
• 通讯作者: Saito Masayuki

Cold Exposure Increases Circulating miR-122 Levels via UCP1-Dependent Mechanism in Mice

寒冷暴露通过 UCP1 依赖性机制增加小鼠循环 miR-122 水平

• DOI: 10.14943/jjvr.68.3.187
• 发表时间: 2020
• 期刊: Japanese Journal of Veterinary Research
• 影响因子: 0.4
• 作者: J.V. Bariuan;Y. Okamatsu-Ogura;A. Tsubota;S. Matsuoka;M. Saito;K. Kimura.
• 通讯作者: K. Kimura.

寒冷暴露は褐色脂肪の熱産生タンパク質 UCP1依存的に血中 miR- 122を上昇させる

冷暴露以棕色脂肪产热蛋白 UCP1 依赖性方式增加血液 miR-122

• 发表时间: 2020
• 作者: Jussiaea Valente Bariuan;岡松優子;松岡慎也;坪田あゆみ;斉藤昌之;木村和弘
• 通讯作者: 木村和弘

Circulating microRNA-122 (miR-122): Do adipose tissues really controbute?

循环 microRNA-122 (miR-122)：脂肪组织真的有影响吗？

• 发表时间: 2018
• 作者: Jussiaea Valente Bariuan;岡松優子;松岡慎也;坪田あゆみ;斉藤昌之;木村和弘;Jussiaea Valente Bariuan;Jussiaea Valente BARIUAN
• 通讯作者: Jussiaea Valente BARIUAN