SFB 394: Structures and Mechanisms in Biological Signal and Energy Transduction

394合作研究中心的目标是确定蛋白质的三维结构，以便在原子水平上描述功能和动态运动。一个特殊的方面是不同蛋白质的结构域相互作用的描述。因此，应解释结构肌肉蛋白质及其调节或膜蛋白质的离子转运或磷酸化氨基酸与蛋白质的相互作用的力发展。将采用一系列方法，如蛋白质原位成像、傅立叶变换红外光谱、电子共振光谱、瞬态动力学、通过“表面等离子体共振”和微量热法实时测量相互作用。此外，激光镊子将与单荧光偏振检测相结合，这将允许在单分子水平上确定不同蛋白质结构域的相互作用。合作研究中心由21个项目组成，其中16个位于鲁尔大学波鸿，包括两个通过质谱法进行肽分析和DNA分析的中心服务项目，5个位于多特蒙德的马克斯普朗克分子生理学研究所。

The goal of the collaborative research centre 394 is to determinethree-dimensional structures of proteins in order to describe function and dynamic movements on an atomic level. A special aspect is the description of the domain interactions of different proteins. Thus force development by structural muscle proteins and their regulation or iontransport by membrane proteins or interactions of phosphorylated amino acids with proteins shall be explained. An array of methods will be employed like proteincristallography, fouriertransforminfraredspectroscopy, electronresonancespectroscopy, transient kinetics, realtime measurements of interactions by "surface plasmon resonance" and microcalorimetry. In addition laserforceps will be developed combined with a single fluorophor-polarisation detection which will allow to determine interactions of domains of different proteins on a single-molecule level. The collaborative research centre is composed of 21 projects of which 16 are located at the Ruhr-Universität Bochum including two central service projects for peptide analysis by masspectrometry and DNA analysis and 5 are located at the Max-Planck-Institut for Molecular Physiology at Dortmund.