大豆miR394靶位点F-box基因Glyma08g11030在干旱胁迫应答中的功能研究

F-box蛋白参与植物对干旱胁迫的响应。研究发现某些F-box蛋白在转录后水平受miRNA的调控。前期工作发现过表达大豆gma-MIR394a明显提高转基因拟南芥的抗旱性，gma-miR394a直接切割F-box基因Glyma08g11030的mRNA分子，推测gma-miR394a在转录后水平通过调控Glyma08g11030基因的表达来响应干旱胁迫。本项目分析Glyma08g11030基因在多种胁迫下和不同组织中的表达模式；对转Glyma08g11030基因拟南芥和大豆植株进行抗旱性鉴定；测定逆境相关的生理指标，了解转Glyma08g11030基因植株响应干旱的生理机制；筛选受Glyma08g11030调控的下游基因和互作的候选蛋白，探讨Glyma08g11030参与的干旱胁迫信号通路中的上下游组分及应答干旱胁迫的分子机制，为揭示大豆miRNAs参与的干旱胁迫应答调控网络提供理论依据。

F-box protein have an important function in adaptive responses to drought stresses. According to our results as well as those of other studies, the regulation of certain F-box protein by miRNA in the post-transcriptional level.In a previous work, overexpression of gma-MIR394a enhanced drought tolerance in transgenic Arabidopsis thaliana, and 5' RACE experiments demonstrated that gma-miR394a directs the cleavage of F-box (Glyma08g11030) mRNA molecules. Therefore, we speculate gma-miR394a at the post-transcriptional level in response to drought stress by regulating the expression of genes Glyma08g11030.In this study, quantitative real-time PCR and promoter:GUS fusions were used to analyze the expression patterns of F-box gene in various tissues and in response to various stresses;Transgenic Arabidopsis and soybean plants of Glyma08g11030 are drought resistance identification; measuring stress-related physiological indicators, to understand transgenic plants respond to drought physiological mechanisms. Downstream genes regulated by Glyma08g11030 and its interacting candidate proteins were screened, to explore the Glyma08g11030 involved in drought stress signaling pathway up/downstream components and of the molecular mechanisms of it responses to drought stress. For revealing soybean miRNAs involved in the regulation of drought stress response network to provide theoretical basis.