Molecular characterization of duckweeds at the level of clones, species, genera and subfamily by amplified fragment length polymorphism and plastidic DNA sequencing

浮萍是一种小型植物（37种），在生物监测（自2006年以来存在ISO协议）和植物修复中具有越来越重要的意义。由于这些植物的大多数特征大大减少，即使对专家来说，在形态学水平上鉴定物种也是一项挑战。因此，分子标记应该得到开发和应用。通过扩增片段长度多态性（AFLP）和对三个可塑DNA区域（tmL - trnF， tmS - trnG和rp/16）的测序，将从所有属和种的浮萍组中获得大约150个已确认身份的克隆，以达到以下目标：建立一种有效的方法来确定有关该物种的新分离植物样品的身份或确认其身份。(2)。浮萍克隆的分子鉴定（条形码）在生物监测、植物修复和遗传转化或克隆鉴定中的应用。(3)。应用所获资料，以检验和完善现有的阿拉科-柠檬科系统发育关系的认识。

Duckweeds (Araceae-Lemnoideae) are a small group of plants (37 species) with increasing importance in biomonitoring (since 2006 an ISO protocol exists) and phytoremediation. Because of the great reduction of most characteristics of these plants, identification of species on a morphological level is a challenge even for specialists. Therefore, molecular markers should be developed and applied. Approximately 150 clones with confirmed identity from all genera and species of the duckweed group will be characterised by amplified fragment length polymorphism (AFLP) and by sequencing three plastidic DNA regions (tmL - trnF, tmS - trnG, and rp/16) to reach the following goals: (1.) Development of an effective method to determine the identity of newly isolated plant samples concerning the species or to confirm their identity. (2.) Molecular characterisation (bar coding) of duckweed clones for application in biomonitoring, phytoremediation and genetic transformation or to confirm their clonal identity. (3.) Application of the obtained data in order to test and improve the present knowledge of phylogenetic relationships in Araceae-Lemnoideae.