Molecular characterization of duckweeds at the level of clones, species, genera and subfamily by amplified fragment length polymorphism and plastidic DNA sequencing

通过扩增片段长度多态性和质体 DNA 测序在克隆、种、属和亚科水平上对浮萍进行分子表征

• 批准号: 56849529
• 负责人: Privatdozent Dr. Klaus-Jürgen Appenroth
• 金额: --
• 依托单位: Matthias-Schleiden-Institut für Genetik, Bioinformatik und Molekulare Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/56849529?language=en
• 关键词: Molecular; characterization; duckweeds; level; clones

Duckweeds (Araceae-Lemnoideae) are a small group of plants (37 species) with increasing importance in biomonitoring (since 2006 an ISO protocol exists) and phytoremediation. Because of the great reduction of most characteristics of these plants, identification of species on a morphological level is a challenge even for specialists. Therefore, molecular markers should be developed and applied. Approximately 150 clones with confirmed identity from all genera and species of the duckweed group will be characterised by amplified fragment length polymorphism (AFLP) and by sequencing three plastidic DNA regions (tmL - trnF, tmS - trnG, and rp/16) to reach the following goals: (1.) Development of an effective method to determine the identity of newly isolated plant samples concerning the species or to confirm their identity. (2.) Molecular characterisation (bar coding) of duckweed clones for application in biomonitoring, phytoremediation and genetic transformation or to confirm their clonal identity. (3.) Application of the obtained data in order to test and improve the present knowledge of phylogenetic relationships in Araceae-Lemnoideae.

浮萍（Araceae-Lemnoideae）是一小群植物（37种），在生物监测（自2006年以来，ISO协议存在）和植物修复中越来越重要。由于这些植物的大多数特征大大减少，即使对专家来说，在形态学水平上识别物种也是一个挑战。因此，应大力发展和应用分子标记。通过扩增片段长度多态性（AFLP）和三个质体DNA区域（tmL-trnF、tmS-trnG和rp/16）的测序来表征来自浮萍组的所有属和种的大约150个具有确认的同一性的克隆，以达到以下目标：开发一种有效的方法来确定新分离的植物样品的身份，或确认它们的身份。（2.）浮萍克隆的分子特征（条形码），用于生物监测，植物修复和遗传转化或确认其克隆身份。（3.）应用所获得的数据，以测试和改善目前的知识在花生科-浮萍亚科的系统发育关系。