Light-induced gene-specific expression of the Lhc gene family in Arabidopsis thaliana and their regulation by transcription and translation

拟南芥Lhc基因家族的光诱导基因特异性表达及其转录和翻译调控

• 批准号: 62071975
• 负责人: Privatdozent Dr. Klaus-Jürgen Appenroth
• 金额: --
• 依托单位: Matthias-Schleiden-Institut für Genetik, Bioinformatik und Molekulare Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/62071975?language=en
• 关键词: Light; induced; gene; specific; expression

Fitness of plants essentially depends on two factors: how effectively they harvest and use light energy, and how successfully they adapt to changing light environment. The adjustment of the photosynthetic light harvesting antenna (LHC) in response to different growth light quantities and qualities is an example of such adaptation. The antenna is composed of different types of chlorophylland carotenoid-binding proteins, the Lhc proteins. In Arabidopsis, Lhc proteins are encoded by a nuclear gene family containing 20 evolutionary related genes that are known to be regulated by light. By application of suitable light conditions combined with the investigation of mutants we want to find out how photosensory processes (mediated by phytochromes) and photosynthesisoriginated signals influence different regulation levels of Lhc genes during development of Arabidopsis seedlings. For the first time, all 20 Lhc genes will be detected specifically under shade conditions in order to establish their distinct functions in shade tolerance physiology. The specific detection is possible by newly developed oligonucleotide hybridization probes. These probes will be used to analyse transcript levels, transcript stability, and translation rate (polyribosome loading) of all of the 20 genes.

植物的适应性主要取决于两个因素：它们如何有效地收集和利用光能，以及它们如何成功地适应不断变化的光环境。响应于不同生长光量和质量的光合光捕获天线（LHC）的调整是这种适应的一个例子。触角是由不同类型的叶绿素和类胡萝卜素结合蛋白，Lhc蛋白。在拟南芥中，Lhc蛋白由一个核基因家族编码，该家族包含20个已知受光调控的进化相关基因。通过应用适宜的光照条件并结合突变体的研究，我们希望了解在拟南芥幼苗发育过程中光敏色素介导的光感受过程和光合信号是如何影响Lhc基因不同的调控水平的。这是第一次，所有20个Lhc基因将在遮荫条件下进行特异性检测，以确定其在耐荫生理中的独特功能。新开发的寡核苷酸杂交探针可用于特异性检测。这些探针将用于分析所有20个基因的转录水平、转录稳定性和翻译速率（多核糖体加载）。