Nanopartikels Containing Selective Probes: Interaction Mechanisms of Nanoparticles with Cell Membranes, Intracellular Uptake, and Transport (NANO-SELECT)

进一步开发了关于有意制造的可变尺寸、形状和表面功能化的纳米颗粒的摄取的协同研究的研究计划。荧光、磁性和放射性探针用于标记纳米颗粒。这用于探测进入细胞、皮肤和整个生物体的摄取途径。这些颗粒是通过胶体化学定制的，其特性通过标准和非标准单颗粒方法表征。颗粒界面是特别感兴趣的，其中吸收到生物介质中引起的表面性质的变化是系统地研究在一个协作和跨学科的努力，涉及研究小组从物理化学，皮肤病学，肿瘤研究和实验室动物科学。具体来说，通过细胞生物学和生物化学方法研究单细胞的内吞作用。研究了纳米颗粒通过皮肤的渗透以及积聚到肿瘤细胞中。通过各种光谱显微镜方法，包括X射线显微镜，显微放射自显影，以及荧光和拉曼显微镜选择性地检测颗粒。联合研究工作的目的是大大有助于纳米颗粒吸收到活的生物体，其中细胞模型系统，皮肤，肿瘤细胞，和生物体中的颗粒分布的机制的理解。

The research program on concerted studies on the uptake of intentionally-made nanoparticles of variable size, shape, and surface functionalization is further developed. Fluorescence, magnetic, and radioactive probes are used for labeling the nanoparticles. This serves for probing the uptake routes into cells, skin, and the entire organism. The particles are custom-made by colloidal chemistry and their properties are characterized by standard and non-standard single-particle approaches. The particle interface is of specific interest, where changes in surface properties induced by uptake into biological media is systematically investigated in a collaborative and interdisciplinary effort involving research groups from physical chemistry, dermatology, tumor research, and laboratory animal sciences. Specifically, endocytosis into single cells is studied by cell biological and biochemical approaches. The penetration of nanoparticles through skin is studied as well as the accumulation into tumor cells. The particles are selectively detected via various spectromicroscopy approaches including X-ray microscopy, microautoradiography, as well as fluorescence and Raman microscopy. Joint research efforts aim to contribute significantly to the understanding of the mechanisms of nanoparticle uptake into living organisms, where cellular model systems, skin, tumor cells, and particle distributions in organisms are investigated.