Investigation of long non-coding RNA signatures of chemoresistance mesothelioma

恶性间皮瘤是一种侵袭性癌症。间皮瘤患者接受顺铂联合培美曲塞化疗并未改善预后，这是由于恶性间皮瘤获得了化疗耐药，目前尚不完全清楚。最近研究发现，长的非编码RNA参与了耐药机制的研究。然而，间皮瘤细胞的作用机制尚不清楚，我们利用全基因表达分析方法分析了化疗耐药间皮瘤和化疗敏感间皮瘤的基因表达。总RNA表达后，将样品在基因芯片上杂交，并用转录组分析软件对数据进行分析。我们发现209个非编码基因在两组间皮瘤中差异表达2倍，其中78个基因上调，131个基因下调，进一步的分析正在进行中。

Malignant mesothelioma is the aggressive cancer. Cisplatin plus pemetrexed chemotherapy in mesothelioma patients has not improved the outcome, due to acquisition of chemoresistance by malignant mesothelioma, which is not yet fully known. Recently, long non-coding RNAs are reported to be involved in chemoresistance mechanism. However, the mechanism in mesothelioma cell is not yet clear.We analyzed gene expression of chemoresistant and chemosensitive mesothelioma using whole gene expression analysis.After Total RNA expression, the samples were hybridized in Genechip and data obtained from the genechip scanner was analyzed with transcriptome Analysis ConsloeWe found 2 fold differential expression of 209 noncoding gene between two groups, with 78 upregulated and 131 downregulated in chemoresistant mesothelioma.Further analysis is ungoing.