Investigation of long non-coding RNA signatures of chemoresistance mesothelioma

化疗耐药间皮瘤的长非编码 RNA 特征的研究

• 批准号: 22K06980
• 负责人: AMATYA VISHWA・J
• 金额: $ 2.66万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K06980/
• 关键词: Mesothelioma; Chemoresistant; lncRNA; Microarray; mesothelioma; lncRNA expression; chemoresistant; microarray; cell lines

Malignant mesothelioma is the aggressive cancer. Cisplatin plus pemetrexed chemotherapy in mesothelioma patients has not improved the outcome, due to acquisition of chemoresistance by malignant mesothelioma, which is not yet fully known. Recently, long non-coding RNAs are reported to be involved in chemoresistance mechanism. However, the mechanism in mesothelioma cell is not yet clear.We analyzed gene expression of chemoresistant and chemosensitive mesothelioma using whole gene expression analysis.After Total RNA expression, the samples were hybridized in Genechip and data obtained from the genechip scanner was analyzed with transcriptome Analysis ConsloeWe found 2 fold differential expression of 209 noncoding gene between two groups, with 78 upregulated and 131 downregulated in chemoresistant mesothelioma.Further analysis is ungoing.

恶性间皮瘤是侵袭性癌症。顺铂加培美曲塞化疗在间皮瘤患者中没有改善结果，因为恶性间皮瘤获得了耐药性，这一点尚未完全了解。近年来，长链非编码RNA被报道参与了化疗耐药机制。本研究采用全基因表达分析方法，对化疗耐药和化疗敏感的间皮瘤细胞进行基因表达分析，将总RNA表达后的标本在基因芯片上进行杂交，用转录组分析软件进行分析，发现两组间有209个非编码基因表达差异2倍，在化疗耐药的间皮瘤中，78例上调，131例下调。进一步的分析正在进行中。