Investigation of long non-coding RNA signatures of chemoresistance mesothelioma

恶性间皮瘤是一种侵袭性癌症。顺铂联合培美曲塞化疗并没有改善间皮瘤患者的预后，因为恶性间皮瘤获得了化疗耐药，这一点尚不完全清楚。近年来，长链非编码rna被报道参与化学耐药机制。然而，在间皮瘤细胞中的作用机制尚不清楚。我们采用全基因表达分析方法分析化疗耐药和化疗敏感间皮瘤的基因表达。总RNA表达完成后，将样品在genchip中进行杂交，并使用转录组分析软件对基因芯片扫描仪获得的数据进行分析。结果发现，在化疗耐药间皮瘤中，两组间209个非编码基因的表达差异为2倍，其中78个表达上调，131个表达下调。进一步的分析正在进行中。

Malignant mesothelioma is the aggressive cancer. Cisplatin plus pemetrexed chemotherapy in mesothelioma patients has not improved the outcome, due to acquisition of chemoresistance by malignant mesothelioma, which is not yet fully known. Recently, long non-coding RNAs are reported to be involved in chemoresistance mechanism. However, the mechanism in mesothelioma cell is not yet clear.We analyzed gene expression of chemoresistant and chemosensitive mesothelioma using whole gene expression analysis.After Total RNA expression, the samples were hybridized in Genechip and data obtained from the genechip scanner was analyzed with transcriptome Analysis ConsloeWe found 2 fold differential expression of 209 noncoding gene between two groups, with 78 upregulated and 131 downregulated in chemoresistant mesothelioma.Further analysis is ungoing.