Contractile forces during cancer cell migration in a 3-D connective tissue matrix

癌细胞在 3D 结缔组织基质中迁移过程中的收缩力

• 批准号: 59573453
• 负责人: Professor Dr. Ben Fabry
• 金额: --
• 依托单位: Peter-Debye-Institut für Physik der weichen Materie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/59573453?language=en
• 关键词: Contractile; forces; during; cancer; cell

The general aim of the proposed project is to characterize the mechanical forces that cancer cells generate during their migration through a 3-dimensional connective tissue matrix. We propose two specific aims. The first aim is to develop a method for measuring the tractions that single cells exert on their surroundings during migration in a connective tissue matrix. The second aim is to test the hypothesis that cancer cell migration in a connective tissue matrix is augmented by the ability to generate higher forces. We will analyze the relationship between force generation and tumor cell invasion as a function of matrix stiffness, matrix protein concentration, mesh-size of the matrix scaffold, and pericellular proteolysis. These data will help us to understand the role of force generation for tumor invasion, and may help explain tumor-specific differences regarding invasiveness and tissue preferences during metastasis formation.

拟议项目的总体目标是表征癌细胞在通过三维结缔组织基质迁移期间产生的机械力。我们提出两个具体目标。第一个目的是开发一种方法来测量牵引力，单细胞在结缔组织基质迁移过程中施加在周围环境。第二个目的是检验癌细胞在结缔组织基质中的迁移通过产生更高力的能力而增强的假设。我们将分析力的产生和肿瘤细胞侵袭之间的关系，作为基质刚度，基质蛋白浓度，基质支架的网格尺寸和细胞周围蛋白水解的函数。这些数据将帮助我们理解力产生对肿瘤侵袭的作用，并可能有助于解释在转移形成期间关于侵袭性和组织偏好的肿瘤特异性差异。

项目成果

Labeling of cancer cells with magnetic nanoparticles for magnetic resonance imaging

• DOI: 10.1002/mrm.24832
• 发表时间: 2014-05
• 期刊: Magnetic Resonance in Medicine
• 影响因子: 3.3
• 作者: C. Weis;F. Blank;A. West;G. Black;R. Woodward;Matthew R J Carroll;A. Mainka;René Kartmann;A. Brandl;H. Bruns;Elizabeth Hallam;J. Shaw;J. Murphy;W. Y. Teoh;K. Aifantis;R. Amal;M. House;Tim St Pierre;B. Fabry

Strain history dependence of the nonlinear stress response of fibrin and collagen networks

• DOI: 10.1073/pnas.1222787110
• 发表时间: 2013-07-23
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Muenster, Stefan;Jawerth, Louise M.;Weitz, David A.
• 通讯作者: Weitz, David A.