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Gingival vaccination strategy to induce both humoral and cellular immunity ideal for elderly vaccination

诱导体液和细胞免疫的牙龈疫苗接种策略非常适合老年人疫苗接种

基本信息

批准号：
22K09932
负责人：
Cueno Marni
金额：
$ 2.75万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2022
资助国家：
日本
起止时间：
2022-04-01 至 2025-03-31
项目状态：
未结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K09932/
关键词：
protein modelling SARS-CoV-2 influenza hemagglutinin vaccine SARS CoV 2 Influenza

项目摘要

We were able to produce the molecular structure of one of the protein antigens that would be used throughout the study. More specifically, the SARS-CoV-2 spike protein from the original, alpha, beta, gamma, and delta variants were successfully designed in silico. We were able to publish our preliminary results related to the in silico design of the SARS-CoV-2 in a peer-reviewed journal. At present, we are likewise designing molecular structures related to the influenza A H3N2 and influenza B/Yamagata hemagglutinin (HA) proteins. Additionally, we also decided to add additional proteins for molecular structure design, namely: SARS-CoV-2 omicron spike and influenza A H5N1 HA proteins since the on-going pandemic is mainly associated with the omicron variant while the on-going bird flu epidemic might have the potential to cross to humans. Protein structures that have already been designed are currently undergoing epitope screening to identify potential B- and T-cell-related conformational epitopes associated with immune response. Moreover, we were also able to perform and confirm xanthan gel and antigen docking involving some of the SARS-CoV-2 spike proteins. We are currently confirming whether epitopes associated with activating B- and T-cell immune responses are readily exposed after xanthan gel molecule docking.

我们能够制造出其中一种蛋白质抗原的分子结构，这将在整个研究中使用。更具体地说，来自原始、阿尔法、贝塔、伽马和德尔塔变种的SARS-CoV-2刺突蛋白是在硅胶中成功设计的。我们能够在一份同行评议的期刊上发表与SARS-CoV-2的电子设计相关的初步结果。目前，我们同样在设计与甲型H3N2型流感和乙型流感/山形血凝素(HA)蛋白相关的分子结构。此外，我们还决定增加用于分子结构设计的额外蛋白质，即：SARS-CoV-2 Omicron Spike和甲型H5N1流感H5N1 HA蛋白质，因为正在进行的大流行主要与Omicron变种有关，而正在进行的禽流感疫情可能会传染给人类。已经设计的蛋白质结构目前正在进行表位筛选，以确定与免疫反应相关的潜在B细胞和T细胞相关构象表位。此外，我们还能够进行和确认涉及一些SARS-CoV-2刺突蛋白的黄原胶和抗原对接。我们目前正在确认与激活B细胞和T细胞免疫反应相关的表位在黄原胶分子对接后是否容易暴露。