Gingival vaccination strategy to induce both humoral and cellular immunity ideal for elderly vaccination

我们能够产生一种蛋白质抗原的分子结构，这种蛋白质抗原将在整个研究中使用。更具体地说，SARS-CoV-2刺突蛋白的原始，α，β，γ和δ变体是成功地设计在电脑上。我们能够在同行评审期刊上发表我们与SARS-CoV-2的计算机设计相关的初步结果。目前，我们同样正在设计与甲型流感H3 N2和B/Yamagata血凝素（HA）蛋白相关的分子结构。此外，我们还决定添加额外的蛋白质用于分子结构设计，即：SARS-CoV-2 omicron spike和甲型流感H5 N1 HA蛋白，因为正在进行的大流行主要与omicron变体相关，而正在进行的禽流感疫情可能有可能交叉到人类。已经设计的蛋白质结构目前正在进行表位筛选，以鉴定与免疫应答相关的潜在B细胞和T细胞相关构象表位。此外，我们还能够执行和确认黄原胶凝胶和抗原对接涉及一些SARS-CoV-2刺突蛋白。我们目前正在确认与激活B-和T-细胞免疫应答相关的表位是否在黄原胶凝胶分子对接后容易暴露。

We were able to produce the molecular structure of one of the protein antigens that would be used throughout the study. More specifically, the SARS-CoV-2 spike protein from the original, alpha, beta, gamma, and delta variants were successfully designed in silico. We were able to publish our preliminary results related to the in silico design of the SARS-CoV-2 in a peer-reviewed journal. At present, we are likewise designing molecular structures related to the influenza A H3N2 and influenza B/Yamagata hemagglutinin (HA) proteins. Additionally, we also decided to add additional proteins for molecular structure design, namely: SARS-CoV-2 omicron spike and influenza A H5N1 HA proteins since the on-going pandemic is mainly associated with the omicron variant while the on-going bird flu epidemic might have the potential to cross to humans. Protein structures that have already been designed are currently undergoing epitope screening to identify potential B- and T-cell-related conformational epitopes associated with immune response. Moreover, we were also able to perform and confirm xanthan gel and antigen docking involving some of the SARS-CoV-2 spike proteins. We are currently confirming whether epitopes associated with activating B- and T-cell immune responses are readily exposed after xanthan gel molecule docking.