权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

骨転移研究のためのマイクロ流体環流システムを用いた３次元骨髄微小環境の構築

利用微流控灌注系统构建3D骨髓微环境用于骨转移研究

基本信息

批准号：
13F03049
负责人：
竹内昌治
金额：
$ 1.47万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2013
资助国家：
日本
起止时间：
2013-04-01 至 2015-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13F03049/
关键词：
3D cell culture Tissue engineering Cancer cell Microfluidic Mesenchymal stem cells Cell fiber 3D cell Culture tissue engineering microfluidics

项目摘要

The main purpose of this research is to engineer the bone marrow niche microenvironment in vitro for metastatic cancer studies using micro-technologies. In the original plan, we proposed to include cancer cells, osteoblasts, and endothelial cells in the system. During the process, we learned about the importance of mesenchymal stem cells and pursued to create three-dimensional (3D) microenvironments from mesenchymal stem cells (MSCs). Initially, multipotent dedifferentiated fat (DFAT) cells that possess similar differentiation abilities as MSCs were used. DFAT cells were encapsulated in the core region of alginate shell fibers using a microfluidic double co-axial device to make 3D cell fiber constructs and differentiated into the smooth muscle cell lineage. Subsequently MSCs derived from adipose tissue, adipose-derived stem cells (ADSCs), were cultured as cell fiber constructs using the same cell fiber technology and differentiated into adipocytes. The conditioned media from the differentiated ADSC fibers was further used to culture MCF-7 breast cancer cells to study its effect on breast cancer cell proliferation.

本研究的主要目的是利用微技术在体外构建骨髓微环境，用于转移性癌症的研究。在最初的计划中，我们建议在系统中包括癌细胞，成骨细胞和内皮细胞。在此过程中，我们了解到间充质干细胞的重要性，并致力于从间充质干细胞（MSC）中创建三维（3D）微环境。最初，使用具有与MSC相似的分化能力的多能去分化脂肪（DFAT）细胞。使用微流体双同轴装置将DFAT细胞包封在藻酸盐壳纤维的核心区域中以制备3D细胞纤维构建体并分化成平滑肌细胞谱系。随后，使用相同的细胞纤维技术将源自脂肪组织的MSC（脂肪源性干细胞（ADSC））培养为细胞纤维构建体并分化成脂肪细胞。进一步将分化的ADSC纤维的条件培养基用于培养MCF-7乳腺癌细胞，以研究其对乳腺癌细胞增殖的影响。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Human adipose-derived stem cell fiber for breast reconstruction

用于乳房重建的人类脂肪干细胞纤维

DOI：
发表时间：
2015
期刊：
IEEE/LEOS International Conference on Optical MEMS
影响因子：
0
作者：
A. Hsiao;T. Okitsu;S. Takeuchi
通讯作者：
S. Takeuchi

Cell fiber technology for engineering functional 3D models

用于工程功能 3D 模型的细胞纤维技术

DOI：
发表时间：
2014
期刊：
影响因子：
0
作者：
Amy Y. Hsiao;Hiroaki Onoe;Teru Okitsu;Midori Kato-Negishi;Shoji Takeuchi
通讯作者：
Shoji Takeuchi

Smooth muscle-like tissue constructs with circumferentially oriented cells formed by the cell fiber technology.

DOI：
10.1371/journal.pone.0119010
发表时间：
2015
期刊：
PloS one
影响因子：
3.7
作者：
Hsiao AY;Okitsu T;Onoe H;Kiyosawa M;Teramae H;Iwanaga S;Kazama T;Matsumoto T;Takeuchi S
通讯作者：
Takeuchi S

3D fiber-shaped culture system promotes differentiation of multipotent DFAT cells into smooth muscle-like cells

3D纤维状培养系统促进多能DFAT细胞分化为平滑肌样细胞