骨転移研究のためのマイクロ流体環流システムを用いた３次元骨髄微小環境の構築

这项研究的主要目的是利用微技术在体外为转移癌研究设计骨髓微环境。在最初的计划中，我们建议将癌细胞、成骨细胞和内皮细胞包括在系统中。在这个过程中，我们了解了间充质干细胞的重要性，并致力于利用间充质干细胞(MSCs)创造三维(3D)微环境。最初，使用了具有与MSCs相似分化能力的多潜能去分化脂肪(DFAT)细胞。利用微流控双同轴仪将DFAT细胞包裹在藻酸盐壳纤维的核心区，构建成3D细胞纤维，并分化为平滑肌细胞谱系。随后，脂肪组织来源的MSCs，脂肪来源的干细胞(ADSCs)，使用相同的细胞纤维技术被培养为细胞纤维结构，并分化为脂肪细胞。将分化的ADSC纤维条件培养液进一步用于MCF-7乳腺癌细胞的培养，研究其对乳腺癌细胞增殖的影响。

The main purpose of this research is to engineer the bone marrow niche microenvironment in vitro for metastatic cancer studies using micro-technologies. In the original plan, we proposed to include cancer cells, osteoblasts, and endothelial cells in the system. During the process, we learned about the importance of mesenchymal stem cells and pursued to create three-dimensional (3D) microenvironments from mesenchymal stem cells (MSCs). Initially, multipotent dedifferentiated fat (DFAT) cells that possess similar differentiation abilities as MSCs were used. DFAT cells were encapsulated in the core region of alginate shell fibers using a microfluidic double co-axial device to make 3D cell fiber constructs and differentiated into the smooth muscle cell lineage. Subsequently MSCs derived from adipose tissue, adipose-derived stem cells (ADSCs), were cultured as cell fiber constructs using the same cell fiber technology and differentiated into adipocytes. The conditioned media from the differentiated ADSC fibers was further used to culture MCF-7 breast cancer cells to study its effect on breast cancer cell proliferation.