骨転移研究のためのマイクロ流体環流システムを用いた３次元骨髄微小環境の構築

本研究的主要目的是利用微技术设计骨髓微环境，用于转移性癌症的体外研究。在最初的计划中，我们建议在系统中包括癌细胞、成骨细胞和内皮细胞。在此过程中，我们了解到间充质干细胞的重要性，并致力于从间充质干细胞（MSCs）中创建三维（3D）微环境。最初，使用具有与MSCs相似分化能力的多能去分化脂肪（DFAT）细胞。采用微流控双同轴装置将DFAT细胞包埋在海藻酸盐壳纤维的核心区域，形成三维细胞纤维结构，并分化为平滑肌细胞系。随后，从脂肪组织中提取的MSCs，即脂肪源性干细胞（ADSCs），使用相同的细胞纤维技术作为细胞纤维构建体进行培养，并分化为脂肪细胞。进一步利用分化后的ADSC纤维条件培养基培养MCF-7乳腺癌细胞，研究其对乳腺癌细胞增殖的影响。

The main purpose of this research is to engineer the bone marrow niche microenvironment in vitro for metastatic cancer studies using micro-technologies. In the original plan, we proposed to include cancer cells, osteoblasts, and endothelial cells in the system. During the process, we learned about the importance of mesenchymal stem cells and pursued to create three-dimensional (3D) microenvironments from mesenchymal stem cells (MSCs). Initially, multipotent dedifferentiated fat (DFAT) cells that possess similar differentiation abilities as MSCs were used. DFAT cells were encapsulated in the core region of alginate shell fibers using a microfluidic double co-axial device to make 3D cell fiber constructs and differentiated into the smooth muscle cell lineage. Subsequently MSCs derived from adipose tissue, adipose-derived stem cells (ADSCs), were cultured as cell fiber constructs using the same cell fiber technology and differentiated into adipocytes. The conditioned media from the differentiated ADSC fibers was further used to culture MCF-7 breast cancer cells to study its effect on breast cancer cell proliferation.