Soil fungi as link between plant derived carbon and soil food webs (FunLink)

本子项目研究真菌在农业土壤中将植物源碳转移到微生物和消费生物中的作用。定植于根和土壤中的丛枝菌根真菌（AMF）代表了菌根（共生）通道。腐养通道是由土壤真菌攻击植物凋落物的复杂碳聚合物形成的。在第一阶段，我们检测到两个通道的真菌分布广泛，它们可能在土壤间充当水平c传递管道。我们还发现了隔室特异性真菌，这表明土壤中的一部分碳转移是通过营养链垂直进行的。新阶段的目的是精确水平和垂直c转移途径的各自重要性，并在垂直途径中考虑微生物内循环的参与。另一个目标是更好地分离植物衍生C通过活的和死的植物材料传递到土壤的各自轨迹。该项目将再次结合现场和微观实验，使用分子工具，如下一代测序和目标RNA和DNA模板，以提高活性真菌生物群的分辨率。我们将利用具有不同生命形式的13C标记真菌菌株实现通用实验模型系统，以更好地描述真菌C在土壤营养链中的轨迹。该SP与SP MicLink、SP NemWeb和SP FaunWeb连接最为紧密。

This subproject investigates the role fungi play in transferring plant-derived carbon to microbial and consumer organisms in agricultural soils. Arbuscular mycorrhizal fungi (AMF) colonizing roots and soils represent the mycorrhizal (symbiotic) channel. The saprotrophic (saprobic) channel is formed by soil fungi attacking complex carbon polymers of the plant litter. In the first phase we detected fungi of both channels with wide distribution, which may act as horizontal C-transfer pipelines between soil compartments. We also found compartment specific fungi, suggesting that a part of the C transfer in soils proceeds vertically through trophic chains. The new phase aims at precising the respective importance of the horizontal and vertical C-transfer routes and, within the vertical one, to consider the involvement of intramicrobial loops. One goal is also to better separate the respective trajectories of the plant derived C delivered to soils by living and by dead plant materials. The project will again combine field and microcosm experiments, use molecular tools such as next generation sequencing and target both RNA and DNA templates to increase the resolution of the active fungal biota. We will contribute to implement common experimental model systems using 13C labelled fungal strains with different life forms to better depict the fungal C trajectory through soil trophic chains. Most strongly this SP is connected to SP MicLink, SP NemWeb and SP FaunWeb.