Plasmonic nanofocusing for super-resolution DNA imaging

用于超分辨率 DNA 成像的等离子体纳米聚焦

• 批准号: DE150101637
• 负责人: Dr Cameron Smith
• 金额: --
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-09-30 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE150101637
• 关键词: Plasmonic; nanofocusing; super; resolution; DNA

Plasmonics (waves in a metal's electrons) can focus light to extreme concentrations that enable imaging techniques to resolve features well beyond the optical barrier known as the diffraction limit. This project aims to develop a routine methodology capable of extracting precise information from single DNA molecules by incorporating plasmonic components into a lab-on-a-chip device for use under conventional optical microscopes. The configuration would have the convenience and technological maturity associated with microscopes whilst being able to capture details of biomolecules with unprecedented detail. New DNA analyses will be made possible by the platform, such as studying the genomic diversity within a population of tumour cells.

等离子体激元（金属电子中的波）可以将光聚焦到极端的浓度，使成像技术能够解决远远超出称为衍射极限的光学屏障的特征。该项目旨在开发一种常规方法，能够通过将等离子体成分纳入芯片实验室设备中，在传统光学显微镜下使用，从单个DNA分子中提取精确信息。该配置将具有与显微镜相关的便利性和技术成熟性，同时能够以前所未有的细节捕获生物分子的细节。该平台将使新的DNA分析成为可能，例如研究肿瘤细胞群体内的基因组多样性。