Overexpression of membrane efflux transporters causing multidrug resistance in field isolates of Botrytis cinerea

膜外排转运蛋白的过度表达导致灰葡萄孢现场分离株的多重耐药性

• 批准号: 66721103
• 负责人: Professor Dr. Matthias Hahn
• 金额: --
• 依托单位: Fachgebiet Phytopathologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/66721103?language=en
• 关键词: Overexpression; membrane; efflux; transporters; causing

In French and German wine-growing regions, increasing Botrytis cinerea populations with multidrug resistance (MDR) phenotypes have been observed. We have identified mutations in MDR strains leading to the overexpression of efflux transporters. In MDR1 strains, gain-offunction mutations in the transcription factor Mrr1 are leading to its activation and thus overexpression of the ABC transporter encoding gene atrB. We also want to investigate the specificity and mechanism of the Mrr1-dependent atrB induction, and to identify the binding site of Mrr1 in the atrB promoter. Using microarray-based transcriptome analyses, we want to identify all Mrr1-regulated genes. In MDR2 strains, two promoter rearrangement mutations caused by retroelement sequences have been identified in the MFS transporter gene mfsM2. We want to confirm the hypothesis that the spread of MDR2 strains has occurred clonally from two ancestor cells, and that one of the mutations have migrated from French into German wine-growing regions. In strawberry fields, we have recently observed a novel and stronger, MDR1-like phenotype (called MDR1*) and we want to identify the underlying mutation(s).

在法国和德国的葡萄酒产区，已经观察到越来越多的具有多药耐药（MDR）表型的葡萄葡萄种群。我们已经在耐多药菌株中发现了导致外排转运蛋白过度表达的突变。在MDR1菌株中，转录因子Mrr1的获得功能突变导致其激活，从而导致编码基因atrB的ABC转运体过表达。我们还想研究Mrr1依赖性atrB诱导的特异性和机制，并确定Mrr1在atrB启动子中的结合位点。利用基于微阵列的转录组分析，我们希望鉴定所有mrr1调控基因。在MDR2菌株中，在MFS转运基因mfsM2中发现了两个由逆转录因子序列引起的启动子重排突变。我们想要证实这样的假设，即MDR2菌株的传播是从两个祖先细胞克隆而来的，其中一个突变已经从法国迁移到德国的葡萄酒产区。在草莓田，我们最近观察到一种新的和更强的MDR1样表型（称为MDR1*），我们想确定潜在的突变。