Flow behaviour of single vesicles and red blood cells in confined geometry disigned by microfluidic channels

微流体通道设计的受限几何结构中单个囊泡和红细胞的流动行为

• 批准号: 69280939
• 负责人: Professor Dr. Thomas Franke
• 金额: --
• 依托单位: College of Science and Engineering
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/69280939?language=en
• 关键词: Flow; behaviour; single; vesicles; red

We plan to study the deformation of single vesicles and red blood cells in hydrodynamic flow fields around them and their mutual interacting with the flow field. The coupling of the shear flow to the deformation and motion of individual soft object gives rise to complex dynamic phenomena such as a rotation of the membrane surface around its inner volume (tank treading), oscillatory (“swinging”) and unsteady (tumbling). In this experimental study we want to characterize these deformations and regimes of motion especially with respect to the viscoe-lastic material properties of soft objects in different shear flow fields. In order to create such flow fields, we intend to design microfluidic channels of varying geometry by soft lithography. The viscoelastic properties of vesicles should be altered by driving the lipid membrane through its temperature-induced fluid-gel transition or to use a binary or ternary mixture of cholesterol and lipids. The latter ones are also known to form domains (“rafts”) of either mi-croscopic or submicroscopic size. In accordance, the cholesterol content of red blood cells is planned to be varied as it is known to be of eminent biological and medical relevance. Furthermore, the deformability of RBCs changes with aging of cells or in diseases such as malaria, hypercholesterolemia and diabetes mellitus .

我们计划研究单个囊泡和红细胞在周围流体动力流场中的变形以及它们与流场的相互作用。剪切流与单个软物体的变形和运动的耦合引起复杂的动态现象，例如膜表面围绕其内部体积的旋转（罐踩踏）、振荡（“摆动”）和不稳定（翻滚）。在这项实验研究中，我们要描述这些变形和运动制度，特别是相对于软物体在不同的剪切流场中的粘滞材料特性。为了创造这样的流场，我们打算通过软光刻设计不同几何形状的微流体通道。囊泡的粘弹性应通过驱动脂质膜通过其温度诱导的流体-凝胶转变或使用胆固醇和脂质的二元或三元混合物来改变。后者也已知形成微观或亚微观尺寸的域（“筏”）。因此，计划改变红细胞的胆固醇含量，因为已知其具有显著的生物学和医学相关性。此外，红细胞的变形性随着细胞的老化或在疾病如疟疾、高胆固醇血症和糖尿病中而改变。