Microorganisms degrading cyclic and short-chain alkanes under anoxic conditions

缺氧条件下降解环状烷烃和短链烷烃的微生物

• 批准号: 71697956
• 负责人: Dr. Florin Musat
• 金额: --
• 依托单位: Helmholtz-Zentrum für Umweltforschung (UFZ)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/71697956?language=en
• 关键词: Microorganisms; degrading; cyclic; short; chain

Propane and butane are important constituents of natural gas and crude oil. Propane and butane reach the biosphere through a worldwide estimate of 10,000 natural oil and gas seeps, or as a result of industrial activities. They play a significant role in carbon cycling at such seepage sites, serving as substrates for aerobic and anaerobic microorganisms. While the aerobic degradation of propane and butane has been intensively studied, relatively little is known of the degradation of these alkanes under anoxic conditions. Strain BuS5 is up to now the only pure culture that can degrade propane and butane under anoxic conditions. Strain BuS5 is a mesophilic, sulphate-reducing bacterium, phylogenetically affiliated with the Desulfosarcina-Desulfococcus group of the Deltaproteobacteria. Physiological tests conducted so far have shown that it can use only propane and n-butane as growth substrates. The present project aims at obtaining a deeper understanding of the anaerobic degradation of propane and n-butane using Strain BuS5 as a model organism. The main objectives are (a) annotation and analysis of the genome of Strain BuS5 with a major focus on the alkane activation/degradation enzymes/pathways; the genome will be available through a project by F. Musat and S. Sievert accepted for funding at the Joint Genome Institute (JGI); (b) proteomic analyses of Strain BuS5 grown with short-chain alkanes; and (c) enzymatic studies aiming at the purification and characterization of the propane- and butane-activating enzyme.

丙烷和丁烷是天然气和原油的重要组分。丙烷和丁烷通过全世界约10，000次天然石油和天然气渗漏或工业活动进入生物圈。它们在这些渗漏点的碳循环中发挥重要作用，作为好氧和厌氧微生物的基质。虽然丙烷和丁烷的好氧降解已被深入研究，但对这些烷烃在缺氧条件下的降解知之甚少。BuS 5菌株是目前唯一一株能在缺氧条件下降解丙烷和丁烷的纯培养物。菌株BuS 5是嗜温的硫酸盐还原细菌，在遗传学上隶属于Deltaproteobacteria的脱硫肉瘤-脱硫球菌组。迄今为止进行的生理测试表明，它只能使用丙烷和正丁烷作为生长基质。本项目的目的是获得一个更深入的了解丙烷和正丁烷的厌氧降解菌株BuS 5作为模式生物。主要目标是（a）对菌株BuS 5的基因组进行注释和分析，主要关注烷烃活化/降解酶/途径;基因组将通过F. Musat和S. Sievert接受了联合基因组研究所（JGI）的资助;（B）用短链烷烃生长的菌株BuS 5的蛋白质组学分析;（c）旨在纯化和表征丙烷和丁烷活化酶的酶研究。