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Does α-MSH of Pars Intermedia of Pituitary and Hypothalamus modulate Fos expression in brain of rats induced by IL-1β?

垂体和下丘脑中间部的α-MSH是否调节IL-1β诱导的大鼠脑内Fos表达？

基本信息

批准号：
08680823
负责人：
NISHIYAMA Keiji
金额：
$ 1.6万
依托单位：
Fukushima Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

Hyperalgesia was induced by intracerebroventricular(ICV) administration of a recombinant human Interleukin-1β(IL-1β) of 1pg - 1ng/kg in rats and the effect of IL-1β was inhibited by amelanocyto stimulating hormone (α-MSH). It has been described that an ICV injection over 100ng IL-1β induced Fos expression in the paraventricular nucleus (PVN) of the hypothalamus in rats, but not in lower doses inducing hyperalgesia. Therefore It is important to know where the sensory response to IL-1β is mediated in the central nerves system (CNS). In order to establish the activated part in the CNS by IL-1β, we examined Fos protein expression in the hypothalamus and modulation by α-MSH for the response to IL-1β in rats. Following the ICV injection of 10 pg IL-1β, a hyperalgesia was induced and Fos was expressed in the PVN and the arcuate nucleus (ARC) which contained α-MSH neurons. But IL-1β did not induce Fos expression in pars intermedia (PI) of pituitary gland which is α-MSH secrete organ in the periphery. A ICV pre-injection of 30 ng α-MSH inhibited hyperalgesia and Fos expression in the PVN and the ARC. This observation demonstrated that PVN neurons were activated by IL-1β and α-MSH inhibited these effects of IL-1β in the PVN. Therefore it is possible that α-MSH neurons in the ARC, which contains endogenous α-MSH were activated to inhibit the effects of IL-1β but α-MSH cells in the PI were not committed with this reaction.

脑室注射1pg-1 ng/kg的重组人白细胞介素1β(IL-1β)诱导大鼠痛觉过敏，并用促贫血激素(β)拮抗IL-1α的作用。脑室注射100 ng以上的IL-1β可诱导大鼠下丘脑室旁核Fos的表达，但在较低剂量下不能引起痛觉过敏。因此，重要的是要知道对IL-1β的感觉反应在中枢神经系统(CNS)中的哪里被调节。为了确定IL-1β在中枢神经系统的激活部分，我们检测了大鼠下丘脑Fos蛋白的表达及α-β对IL-1刺激反应的调节作用。侧脑室注射10pg IL-1β后，在下丘脑室旁核和弓状核(含α-mSh神经元)有Fos表达。但IL-1β不能诱导α-MSH外周分泌器官--垂体中间部Fos的表达。侧脑室注射30 ngα-MSH可抑制痛过敏反应和下丘脑室旁核和弓状核Fos的表达。结果表明，IL-1β可激活下丘脑室旁核神经元，α-MSH可抑制IL-1β对下丘脑室旁核神经元的作用。因此，内源性α-mSh在弓状体内的神经元可能被激活以抑制IL-1β的作用，而PI中的α-msh细胞不参与这一反应。