NEURONAL CIRCUIT FORMATION IN THE GENETICAL AND EXPERIMENTAL BRAIN MALFORMATION ANIMAL.

遗传和实验脑畸形动物的神经回路形成。

• 批准号: 08680824
• 负责人: UEDA Shuichi
• 金额: $ 1.54万
• 依托单位: DOKKYO UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680824/
• 关键词: S-100beta; Serotonin; Mutant mouse; Glia; Immunohistochemistry; Growth factor; Raphe nucleus; Cortex; セトロニン; ニュータントマウス; 小頭症; 回路形式

Homozygote of a mouse strain with genetic polydactyly (Pdn) show disrupted cortical lamination and significant decrease of S-100beta immunoreactive elements in a particular area of the brain. In order to understand the abnormal cortical formation at the cellular level, migration of cortical neurons and development of glia cells were studied using bromodeoxyuridine (BrdU), S-100beta and glial fibrillary acidic protein (GFAP) immunohistochemistry. Homozygous mice (Pdn/Pdn) displayd a variable pattern of abnormalities. Irregular GFAP-positive radial glia cells and disturbance of neuronal migration were found in a circumscribed area of the caudo-dorsal cortex of newborn Pdn mouse. The number of S-100beta-positive cells was reduced in this area. The present results suggest that abnormal cortical lamination closely correlates with disturbance of neuronal migration and abnormalities of glia cells, especially a significant decrease of S-100beta immunoreactive cells.

遗传性多指小鼠品系纯合子表现出大脑某一特定区域皮质层层破坏和S-100β免疫反应元件显著减少。为了在细胞水平上了解皮质的异常形成，用溴脱氧尿苷、S-100β和胶质纤维酸性蛋白免疫组织化学方法研究了皮质神经元的迁移和胶质细胞的发育。纯合子小鼠(PDN/PDN)表现出不同的异常模式。新生PDN小鼠尾侧背侧皮质可见不规则的GFAP阳性放射状胶质细胞和神经元迁移障碍。S-100β阳性细胞在该区域减少。提示皮质分层异常与神经元迁移障碍和胶质细胞异常密切相关，尤其与S-100β免疫反应细胞的显著减少密切相关。

项目成果

Yoshimoto, K., Yayama, K., Sorimachi, Y., Tani, J., Uemura, K., Yoshida, T., Ogata, M., Nishimura, A., Ueda, S., Komura, S.: "Effects of ion channel blockers on rapid postmortem changes in extracellular dopamine and serotonin levels in the rat nucleus acc

吉本 K.、矢山 K.、反町 Y.、谷 J.、上村 K.、吉田 T.、绪方 M.、西村 A.、上田 S.、小村 S.：

Ueda,S.: "Organization of regenerating serotonergic fibers in the hippocampal formation." Brain Res Bull.39. 89-99 (1996)

Ueda,S.：“海马结构中再生血清素纤维的组织。”

Yoshimoto, K.: "Possibility of 5-HT_3 receptor involvement in alcohol dependence:A microdialysis study of nucleus accumbens dopamine and serotonin release in rats with chronic alcohol consumption" Alcohol.Clin.Exp.Res.20. 311A-319A (1996)

Yoshimoto, K.：“5-HT_3 受体参与酒精依赖的可能性：慢性饮酒大鼠伏核多巴胺和血清素释放的微透析研究”Alcohol.Clin.Exp.Res.20。

Ueda, S.: "Age-related degeneration of the serotoninergic fibers in the zitter brain" Synapse. (in press).

Ueda, S.：“zitter 大脑中血清素能纤维与年龄相关的退化”突触。

Ueda,S.: "Age-related degeneration of the serotoninergic fibers in the zitter brain" Synapse. (in press).

Ueda,S.：“齐特脑中血清素能纤维与年龄相关的退化”突触。