EFFECTS OF HIGH PRESSURE PULSES ON CELL ORGANELLE AND DIFFERENTIAL CELLS

为了研究高压脉冲对活体的影响，我们研究了冲击波对动物细胞和细胞成分的机械和生理影响。首先，将几种小鼠肿瘤细胞和正常小鼠细胞置于无膈激波管产生的激波下，得到以下结果：1)细胞分为两组，一组较弱，在峰值压力3 ~ 4mpa以上的高压脉冲下被破坏；另一组较强，在峰值压力高达8mpa的高压脉冲下未被破坏。2)细胞对冲击波暴露的耐受性与细胞的粘附性有关，非贴壁细胞较弱，贴壁细胞较强。3)细胞耐受性与细胞的恶性程度无关。4)细胞耐受性与细胞大小和亚细胞结构密度无关。这可能与细胞膜的结构有关。其次，为了阐明细胞耐受性差异的更多用途，我们研究了细胞耐受性与组织中细胞原始状态、细胞分化或细胞突变之间的关系。我们还研究了每个细胞在单个弱细胞群体中是否具有不同的耐受性。结果如下。1)细胞耐受性与细胞大小和亚细胞结构密度无关。2)悬浮增殖细胞和淋巴样细胞较弱，贴壁增殖细胞和结缔组织细胞较强。3)至少在正常成纤维细胞中，细胞耐受性与细胞分裂数之间没有相关性。4)由于破坏细胞的比例不随高压脉冲次数的增加而增加，因此单个弱细胞群体在细胞耐受方面可能是异质的。5)从这个意义上说，有可能一些弱细胞很容易被几个高压脉冲破坏，而另一些细胞则不被破坏，除非增加峰值压力。6)同一细胞群体中这种细胞耐受性的差异可能与每个细胞的细胞周期有关。少

In order to study effects of a high pressure pulse on a living body, we investigated mechanical and physiological effects of a shock wave on animal cells and cellular components. First, several mouse tumor cells and normal mouse cells were exposed to shock waves which were generated by a diaphragmless shock tube and we obtained following results.1)Cells divided two groups, one is weak and destructed by high pressure pulses at over 3 - 4 MPa of peak pressure and the other is relatively strong and not destructed by the high pressure pulses at up to 8 MPa of peak pressure.2)The cellular tolerance against shock wave exposure was related to adhesiveness of the cells, non-adherent cells were were weak and adherent cells were relatively strong.3)The cellular tolerance was not related to malignancy of the cells.4)The cellular tolerance was not related to the cell size nor to the density of subcellular structures. It may be related to the structure of cell membrane.Next, inorder to elucidate ca … More uses of the difference in the cellular tolerance, we investigated relation between the cellular tolerance and original condition of the cells in tissues, cellular differenciation, or cellular mutation. We also investigated whether each cell has different tolerance in a single weak cell population. Followongs are the results.1)The cellular tolerance was not related to the cell size nor to the density of subcellular structures.2)Cells which proliferate in suspension and lymphoid cells were weak, while cells which proliferate in adherent state and conective tissue cells were relatively strong.3)At least in normal fibroblast cells, there was no relation between the cellular tolerance and count of the cell division.4)Since the fraction of destructed cells do not increase with increasing the number of high pressure pulse, a single weak cell population may be heterogeneous with respect to the cellular tolerance.5)In this sense, it is possible that some weak cells are destructed easily by a few high pressure pulses but the other cells are not destructed unless the peak pressure is increased.6)The difference in this cellular tolerance in the same cell population may be related to the cell cycle of each cell. Less