Study on interaction between the photoresponsive dendrimer and biorelative compounds

光响应树枝状聚合物与生物相关化合物相互作用的研究

基本信息

批准号：
09680573
负责人：
TAMAGAKI Seizo
金额：
$ 2.3万
依托单位：
Osaka City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Polyamidoamine dendrimers are a new class of highly branched spherical polymers that are highly soluble in aqueous solution and has a unique surface of primary amino groups. This class of dendrimers have been used successfully as a vehicle for transferring nucleic acids into mammalian cells. On the other hand, we have synthesized and evaluated novel photochromic dendrimers including polyazobenzenes in the branch structure.ィイD1[3]ィエD1 Polyazobenzene dendrimers are phtochemically size-control-lable. If the size-change of cationic polyazobenzene dendrimers induced by UV irradiation cause the alteration of the cation density on the surface, the affinities of these cationic compounds toward polyanionic nucleic acid are expected to be variable.A new water-soluble polyazobenzene dendrimer modified with L-lysines at the periphery (Lys-G2) was examined as a synthetic vector to mammalian cell. The results from light scattering and gel filtration chromatography showed that the particle size is controllable upon UV or visible light irradiation. Furthermore, a gel shift assay with plasmid DNA and an electrophoretic light scattering analysis demonstrated that the affinity of this cationic dendrimer toward DNA is photo-controllable on the basis of zeta potential alteration on dendrimer's surface. Interestingly, UV light irradiation after the incorporation of DNA-complex with Lys-G2 into a cytoplasm significantly caused a fifty-percent increase on the transfection efficiency.

聚氨基氨基聚合物是一类新的高度分支的球形聚合物，在水溶液中高度固体，并且具有主要氨基基团的独特表面。这类树枝状聚合物已成功用作将核酸转移到哺乳动物细胞中的载体。另一方面，我们已经合成并评估了新型的光致变色树状聚合物，包括分支结构中的多氮杂烯。聚唑苯树状聚合物在磷酸化的大小控制上是可控制的。如果由紫外线照射诱导的阳离子多唑苯在树突中的大小变化会导致表面上的阳离子密度的改变，则这些阳离子化合物对多支核酸的相关性预计将是可变的。哺乳动物细胞。光散射和凝胶过滤色谱法的结果表明，粒径是在紫外线或可见光照射下控制的。此外，具有质粒DNA和电泳光散射分析的凝胶转移测定法表明，该阳离子树枝状聚合物对DNA的亲和力是基于ZETA对树枝状聚合物表面的ZETA电势的改变。有趣的是，将DNA-complex与Lys-G2掺入细胞质后的紫外线照射显着导致转化效率提高50％。