Functional role of the ATP receptor-mediated signaling in the solitary network

ATP 受体介导的信号在孤独网络中的功能作用

基本信息

批准号：
09680773
负责人：
KATO Fusao
金额：
$ 1.92万
依托单位：
Jikei University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680773/
关键词：
nucleus tractus solitarii dorsal motor nucleus of vagus brain slice patch-clamp postsynaptic crrent ligand-gated channel adenosine 5'-triphosphate ATP receptor アテノシン5-シリン酸神経伝達パッチ・クランプ神経細胞アデノシン三リン酸

项目摘要

It was attempted to clarify the functional role of extracellular ATP-mediated signaling in the central cardiorespiratory network in the solitary complex in which abundant expression of P2X (ATP-gated non-specific cation channels) has been demonstrated histochemically. The whole-cell current was recorded from neurons visually identified with infra-red videomicroscopy in the thick brainstem slice of rats (10-30 days old). The following conclusions were drawn.1. Involvement of ATP-mediated synaptic transmission in the synapse from solitary tract (TS) to second-order neurons in the nucleus of the solitary tract (NTS) seems to be little. I failed to detect component resistant to CNQX, AP5, bicuculline and strychnine in the TS stimulation-evoked post-synaptic current (PSC). In addition, on contrary to those demonstrated using freshly dissociated NTS cells, no NTS neuron showed inward current with ATP (n=10).2. However, ATP and its analogues modified PSCs evoked by TS stimulation of the second-order NTS neurons, indicating that P2X receptors are involved in presynaptic regulation of the input gain of afferent information.3. About 70% of the large diameter (>*30 mum) neurons in dorsal motor nucleus of vagus (dmnX) showed ATP-induced TTX-resistant inward currents together with burst-like PSCs. Bicuculline abolished most of Recording with CsCl in the patch solution indicated that these are mainly GABAergic and partly glutaminergic PSCs. The PSCs activated by ATP was abolished with TTX.This is a novel finding indicating that, in addition to the parasympathetic preganglionic cells, the GABAergic and glutaminergic premotor neurons sending synaptic connections to these cells also express P2X receptors.

它试图阐明细胞外ATP介导的信号在中心呼吸型网络中的功能作用，在该复合物中，在组织化学上已经证明了P2X（ATP门控的非特异性阳离子通道）的丰富表达（ATP基因非特异性阳离子通道）。全细胞电流是从视觉上用红外视频显微镜识别的神经元记录的，这是大鼠（10-30天大）的脑干切片中的。得出以下结论1。 ATP介导的突触传播参与了孤立区（NTS）核中二阶神经元的突触中的突触传播似乎很少。我未能检测到TS刺激引起的突触后电流（PSC）中对CNQX，AP5，双核和士曲他的抗性成分。此外，与使用新鲜分离的NTS细胞所证明的相反，NTS神经元没有ATP表现出内向电流（n = 10）.2。但是，ATP及其类似物通过TS刺激二阶NTS神经元刺激了PSC，表明P2X受体参与了传入信息的输入增益的突触前调节。3。大约70％的大直径（>*30 MUM）神经元在迷走神经（DMNX）的背运动核中显示ATP诱导的TTX耐药向内电流以及爆发样的PSC。斑块溶液中用CSCL废除了大多数记录，表明这些主要是GABA能和部分谷氨酰胺能PSC。用TTX废除了ATP激活的PSC。这是一个新颖的发现，表明，除了副交感神经前细胞外，GABA能和谷氨酰胺能神经元可向这些细胞发送突触连接，还表达P2X受体。