Functional role of the ATP receptor-mediated signaling in the solitary network

ATP 受体介导的信号在孤独网络中的功能作用

• 批准号: 09680773
• 负责人: KATO Fusao
• 金额: $ 1.92万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680773/
• 关键词: nucleus tractus solitarii; dorsal motor nucleus of vagus; brain slice; patch-clamp; postsynaptic crrent; ligand-gated channel; adenosine 5'-triphosphate; ATP receptor; アテノシン5-シリン酸; 神経伝達; パッチ・クランプ; 神経細胞; アデノシン三リン酸

It was attempted to clarify the functional role of extracellular ATP-mediated signaling in the central cardiorespiratory network in the solitary complex in which abundant expression of P2X (ATP-gated non-specific cation channels) has been demonstrated histochemically. The whole-cell current was recorded from neurons visually identified with infra-red videomicroscopy in the thick brainstem slice of rats (10-30 days old). The following conclusions were drawn.1. Involvement of ATP-mediated synaptic transmission in the synapse from solitary tract (TS) to second-order neurons in the nucleus of the solitary tract (NTS) seems to be little. I failed to detect component resistant to CNQX, AP5, bicuculline and strychnine in the TS stimulation-evoked post-synaptic current (PSC). In addition, on contrary to those demonstrated using freshly dissociated NTS cells, no NTS neuron showed inward current with ATP (n=10).2. However, ATP and its analogues modified PSCs evoked by TS stimulation of the second-order NTS neurons, indicating that P2X receptors are involved in presynaptic regulation of the input gain of afferent information.3. About 70% of the large diameter (>*30 mum) neurons in dorsal motor nucleus of vagus (dmnX) showed ATP-induced TTX-resistant inward currents together with burst-like PSCs. Bicuculline abolished most of Recording with CsCl in the patch solution indicated that these are mainly GABAergic and partly glutaminergic PSCs. The PSCs activated by ATP was abolished with TTX.This is a novel finding indicating that, in addition to the parasympathetic preganglionic cells, the GABAergic and glutaminergic premotor neurons sending synaptic connections to these cells also express P2X receptors.

它试图澄清的功能作用的细胞外ATP介导的信号转导在中央心肺网络中的孤立复合体，其中丰富的表达的P2 X（ATP门控的非特异性阳离子通道）已被证明组织化学。全细胞电流记录在大鼠（10-30日龄）的脑干厚切片中用红外视频显微镜视觉识别的神经元。得出以下结论：1. ATP介导的突触传递在从孤束（TS）到孤束核（NTS）二级神经元的突触中的参与似乎很少。在TS刺激诱发的突触后电流（PSC）中，我未能检测到对CNQX、AP 5、荷包牡丹碱和士的宁有抗性的成分。另外，与新鲜分离的NTS细胞相反，没有NTS神经元在ATP作用下显示内向电流（n=10）.而ATP及其类似物可修饰TS刺激NTS二级神经元所诱发的PSCs，表明P2 X受体参与了传入信息输入增益的突触前调节.迷走神经背侧运动核（dmnX）中约70%的大直径（> 30 μ m）神经元显示ATP诱导的TTX抗性内向电流和爆发样PSC。荷包牡丹碱消除了大部分的记录与氯化铯在补丁的解决方案表明，这些主要是GABA能和部分多巴胺能PSC。这一新的发现表明，除了副交感神经节前细胞外，GABA能和多巴胺能运动前神经元也表达P2 X受体。

项目成果

Kato, F.: "Adenosine 5'-triphosphate induces P2X purinoceptor-and non-NMDA receptor-mediated inward currents of the solitary complex neurons in rat brainstem slice." Soc.Neurosci.Abstr.24. 630.2 (1998)

Kato, F.：“5-三磷酸腺苷可诱导大鼠脑干切片中孤立复杂神经元的 P2X 嘌呤受体和非 NMDA 受体介导的内向电流。”

Masaki, E., Kato, F., Kondo, I.& Kawamura, M.: "P2X purinergic receptors as a possible site of action of sevoflurane." Anesthesiology. 89. A113 (1998)

正树，E.，加藤，F.，近藤，I.

Kato, F.: "Burst-like postsynaptic inward currents evoked by adenosine 5'-triphosphate of the solitary complex neurons in rat brainstem slice." Neurosci.Res.22. S253 (1998)

Kato, F.：“大鼠脑干切片中孤立复合神经元的 5-三磷酸腺苷诱发的突发状突触后内向电流。”

Kato, F.: "Burst-like postsynaptic inward currents evoked by adenosine 5′-triphosphate of the solitary complex neurons in rat brainstem sllce" Neurosci. Res.22. S253 (1998)

Kato, F.：“大鼠脑干中孤立复合神经元的 5-三磷酸腺苷诱发的突发状突触后内向电流”Resci S253 (1998)。

加藤総夫: "ニューロサイエンスの新しい研究方法-赤外線顕微鏡下の脳スライス・パッチクランプ法" クリニカル・ニューロサイエンス. 10. 848-849 (1998)

加藤武夫：“神经科学的新研究方法——红外显微镜下的脑切片/膜片钳方法”《临床神经科学》10. 848-849 (1998)。