Mechanism of the transmitter release facilitation through activation of presynaptic ATP receptors in the brainstem

通过激活脑干突触前 ATP 受体促进递质释放的机制

基本信息

  • 批准号:
    13680902
  • 负责人:
  • 金额:
    $ 1.98万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2002
  • 项目状态:
    已结题

项目摘要

P2X receptors (P2X-Rs) are ATP-gated channels permeable to cations inclining Ca^<2+>. We recorded miniature excitatory postsynaptic currents (mEPSCs) under blockade of voltage-dependent Na^+ channels from second-order neurons in the nucleus tractus solitarii in vitro, a part of the brain where exogenous ATP has profound effects on autonomic outflow. Activation of presynaptic P2X-Rs with ATP or α,β-methylene ATP (α,βmATP) not only enhanced the release of glutamate, but could initiate it de novo even when voltage-dependent calcium channels (VDCCs) were completely blocked. This facilitating effect of αβmATP on the glutamate release was abolished completely by a P2X-R antagonist pyridoxalphosphate-6-azophenyl-2',4' -disulphonic acid (PPADS) and by a high, but not a low, concentration of a subtype-selective antagonist 2 > ,3 ' -O-(2,4,6-trinitrophenyl)-ATP (TNP-ATP). Despite manifest increase in the frequency of excitatory postsynaptic events by αβmATP, the rate of the synaptic failures as evaluated by solitary tract stimulation was not significantly affected. The miniature excitatory postsynaptic potentials elicited with P2X-R agonists recorded under blockade of VDCC-dependent synaptic transmission were sufficient to trigger action potentials in the postsynaptic cell. These results show that a rise in extracellular ATP concentration promotes excitatory transmission even in the absence of presynaptic excitation, leading to a consequent activation of the autonomic networks.
P2 X受体(P2 X-Rs)是一种ATP门控通道,对Ca 2+具有渗透性。我们在体外孤束核二级神经元上记录了电压依赖性Na^+通道阻断后的微小兴奋性突触后电流(mEPSC)。用ATP或α,β-亚甲基ATP(α,βmATP)激活突触前P2 X-Rs不仅能促进谷氨酸的释放,而且即使电压依赖性钙通道(VDCCs)被完全阻断也能重新启动谷氨酸的释放。αβmATP对谷氨酸释放的这种促进作用可被P2 X-R拮抗剂磷酸吡哆醛-6-偶氮苯基-2 ',4' -二磺酸(PPADS)和高浓度(但不是低浓度)的亚型选择性拮抗剂2 >,3 ' -O-(2,4,6-trinitrophenyl)-ATP(TNP-ATP)完全消除。尽管αβmATP明显增加兴奋性突触后事件的频率,但通过孤束刺激评估的突触失败率没有显著影响。在阻断VDCC依赖性突触传递的情况下记录的P2 X-R激动剂引起的微型兴奋性突触后电位足以触发突触后细胞中的动作电位。这些结果表明,细胞外ATP浓度的上升,促进兴奋性传输,即使在突触前兴奋的情况下,导致随后激活的自主神经网络。

项目成果

期刊论文数量(89)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nishi H, Kato F, Masaki E, Kawamura M: "ADP-sensitive purinoceptors induce steroidogenesis via adenylyl cyclase activation in bovine adrenocortical fasciculata cells"Brit J Pharraacol. J137. 177-184 (2002)
Nishi H、Kato F、Masaki E、Kawamura M:“ADP 敏感嘌呤受体通过牛肾上腺皮质束状细胞中的腺苷酸环化酶激活诱导类固醇生成”Brit J Pharraacol。
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    0
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Kawamura M, Masaki E, Kato F: "Regulation of inhibitory synaptic transmission by cholinoceptors in the brainstem nociceptive network of the rat"The 3rd International Symposium on the Study of Brain Function Abstracts. 213 (2002)
Kawamura M、Masaki E、Kato F:“大鼠脑干伤害性网络中胆碱受体对抑制性突触传递的调节”第三届国际脑功能研究研讨会摘要。
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    0
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Kawamura M, Tanaka J. Inoue K, Kato F: "Rat kaiba CA3 suitai saibou heno kohunsei oyobi yokuseisei shinapusu nyuuryoku ni oyobosu saibougai ATP no souhantekisayou (in Japanese)"Shinkei-Kagaku. 40 (2&3). 437 (2001)
Kawamura M、Tanaka J. Inoue K、Kato F:“Rat kaiba CA3suitai saibou heno kohunsei oyobi yokuseisei shinapusu nyuuryoku ni oyobosu saibougai ATP no souhantekisayou(日语)”Shinkei-Kagaku。
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    0
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Kato F: "Kosokukaku ATP-juyotai to kokyuseigyo (in Japanese)"Kokyu-to-Junkan. 50. 43-51 (2002)
加藤 F:“Kosokukaku ATP-juyotai to kokyuseigyo(日语)”Kokyu-to-Junkan。
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  • 影响因子:
    0
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Kato F: ""Hitotsubu de nido oishi" saibou-gai messenger to shiteno ATP - Saibou-gai ATP to purine juyotai niyoru nounai shiiiapusu dentatsu no taju-seigyo (in Japanese)"No-kinou No Kaimei - Seimei Kagaku no Syuchoryuu- eds.Akaike, Higashi, Abe, Kubo.Gaiya
加藤 F:““Hitotsubu de nido oishi”saibou-gai Messenger tooshiten ATP - Saibou-gai ATP to purine juyotai niyoru nounai shiiiapusu dentatsu no taju-seigyo(日文)”No-kinou No Kaimei - Seimei Kagaku no Syuchoryuu-eds
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KATO Fusao其他文献

KATO Fusao的其他文献

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{{ truncateString('KATO Fusao', 18)}}的其他基金

Functional connectomics analysis of synaptic transmission using optogenetics
使用光遗传学进行突触传递的功能连接组学分析
  • 批准号:
    23650208
  • 财政年份:
    2011
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
The historical correlation of self-government and local economy in modern Germany
近代德国自治与地方经济的历史关联
  • 批准号:
    22530340
  • 财政年份:
    2010
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Functional significance of nociceptive amygdala in pain-induced negative emotion
伤害性杏仁核在疼痛引起的负面情绪中的功能意义
  • 批准号:
    21390436
  • 财政年份:
    2009
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The regional economy and local autonomy in Germany and Switzerland
德国和瑞士的区域经济和地方自治
  • 批准号:
    19530310
  • 财政年份:
    2007
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Facilitation of neurotransmittcr release through activation of presynaptic P_2X receptors by endogenous ATP
通过内源性 ATP 激活突触前 P_2X 受体促进神经递质释放
  • 批准号:
    17300123
  • 财政年份:
    2005
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Berlin and the City-land-continuum in Prussia
柏林和普鲁士的城市-土地-连续体
  • 批准号:
    15530238
  • 财政年份:
    2003
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Rural Society in East-Elbe and German Suburban Country Village
东易北河农村社会与德国郊区乡村
  • 批准号:
    10630075
  • 财政年份:
    1998
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Functional role of the ATP receptor-mediated signaling in the solitary network
ATP 受体介导的信号在孤独网络中的功能作用
  • 批准号:
    09680773
  • 财政年份:
    1997
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
German Hereditary Estate and European Rural Society
德国世袭庄园和欧洲农村社会
  • 批准号:
    08630076
  • 财政年份:
    1996
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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