ACTIVITY-DEPENDENT REGULATION OF NEURAL CELL ADHESION MOLECULES, NEUROTROPHINS AND THEIR RECEPTORS IN NEURONS.

神经细胞粘附分子、神经营养因子及其神经元受体的活动依赖性调节。

• 批准号: 09680783
• 负责人: ITOH Kouichi
• 金额: $ 1.79万
• 依托单位: TOKYO METROPOLITAN ORGANIZATION FOR MEDICAL RESEARCH
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680783/
• 关键词: NEURONAL ACTIVITY; NEURAL CELL ADHESION MOLECULES; NEUROTROPHIC FACTORS; trk; DRG NEURONS; CELL CULTURES; MOUSE; ELECTRICAL STIMULATION; Neuron; Plasticity; Cell Adhesion Molecules; LTP; Brain; Nervous System; plasticity; cell adhesion Mole

We studied the relationship between neuronal activity, neural cell adhesion molecules and neurotrophins in mouse dorsal root ganglion (DRG) neurons during development in vitro. The nervous system structure is modified by electrical activity in developing neural circuits, but the molecular mechanisms are not well under stood. Recognition molecules and neurotrophic factors regulate cell interactions during development. Therefore, the possible involvement of neural cell adhesion molecules (L1, NCAM and N-cadherin), and of neurotrophins (NGF, BDNF and NT-3) in activity-development was studied by delivering electrical pulses to mouse DRG neurons in cultures. After 5 days exposure to NGF, but not after exposure to BDNF, the NT-3 and L1 levels increased 3〜4 fold. Low frequency stimulation (0.1 Hz for 5 days) decreased L1 and trkC levels in the absence of NGF. Regulation of the expression of L1 and trkC by specific patterns of impulse activity may influence cell interactions coordinating structure and function of the nervous system at critical developmental stages.

本实验研究了小鼠背根神经节（DRG）神经元发育过程中神经元活动与神经细胞粘附分子和神经营养因子的关系。在神经回路发育过程中，神经系统的结构会受到电活动的影响，但其分子机制还不清楚。识别分子和神经营养因子调节发育过程中的细胞相互作用。因此，可能参与的神经细胞粘附分子（L1，NCAM和N-钙粘蛋白），和神经营养因子（NGF，BDNF和NT-3）的活动发展进行了研究，通过提供电脉冲培养小鼠DRG神经元。在暴露于NGF 5天后，而不是暴露于BDNF后，NT-3和L1水平增加了3 ~ 4倍。低频刺激（0.1 Hz，5天）降低L1和trkC水平的情况下，神经生长因子。通过特定的冲动活动模式调节L1和trkC的表达可能会影响细胞相互作用，协调神经系统在关键发育阶段的结构和功能。

项目成果

H.Asou, Y.Takeda, K.Itoh and K.Uyemura,: "The RSLE sequence in the cytoplasmic domain of adhesion molecule L1 is involved in cell migration on L1 substrate (K. Uyemura, K. Kawamura and T. Yazaki, edts)."Neural Development/Keio Univ Symp for Life Sci and M

H.Asou、Y.Takeda、K.Itoh 和 K.Uyemura，：“粘附分子 L1 胞质结构域中的 RSLE 序列参与 L1 底物上的细胞迁移（K. Uyemura、K. Kawamura 和 T. Yazaki，

R.D.FIELDS: "Action potential-dependent regulation of gene expression : Temporal specificity in Ca++,CREB and MAP kinase signaling." J.Neurosci.17. 7252-7266 (1997)

R.D.FIELDS：“基因表达的动作电位依赖性调节：Ca 、CREB ​​和 MAP 激酶信号传导的时间特异性。”

K.Itoh, M.N.Ozaki, B.Stevens and R.D.Fields,: "Activity-dependent regulation of N-cadherin in DRG neurons: Differential regulation of N-cadherin, NCAM and L1 by distinct patterns of action potentials."J. Neurobiol.. 33. 735-748 (1997)

K.Itoh、M.N.Ozaki、B.Stevens 和 R.D.Fields，：“DRG 神经元中 N-钙粘蛋白的活动依赖性调节：不同动作电位模式对 N-钙粘蛋白、NCAM 和 L1 的差异调节。”

K. Itoh et al.: "Fields, Activity-dependent regulation of N-cadherin in DRG neurons : Differential regulation of N-cadherin, NCAM and L1 by distinct patterns of action potentials"J. Neurobiol.. 33. 735-748 (1997)

K. Itoh 等人：“DRG 神经元中 N-钙粘蛋白的场、活动依赖性调节：通过不同的动作电位模式对 N-钙粘蛋白、NCAM 和 L1 进行差异调节”J.

K.Yoshimura, Y.Sakurai, D.Nishimura, Y.Tsuruo, M.Nomura, K.Kawato, C.Seiwa, T.Iguchi, K.Itoh and H.Asou,: "A monoclonal antibody, 14F7, which recognizes a stage-specific immature oligodendrocyte surface molecule inhibits, oligodendrocyte differentiation m

K.Yoshimura、Y.Sakurai、D.Nishimura、Y.Tsuruo、M.Nomura、K.Kawato、C.Seiwa、T.Iguchi、K.Itoh 和 H.Asou，：“一种单克隆抗体 14F7，可识别