ACTIVITY-DEPENDENT REGULATION OF NEURAL CELL ADHESION MOLECULES, NEUROTROPHINS AND THEIR RECEPTORS IN NEURONS.

本实验研究了小鼠背根神经节（DRG）神经元发育过程中神经元活动与神经细胞粘附分子和神经营养因子的关系。在神经回路发育过程中，神经系统的结构会受到电活动的影响，但其分子机制还不清楚。识别分子和神经营养因子调节发育过程中的细胞相互作用。因此，可能参与的神经细胞粘附分子（L1，NCAM和N-钙粘蛋白），和神经营养因子（NGF，BDNF和NT-3）的活动发展进行了研究，通过提供电脉冲培养小鼠DRG神经元。在暴露于NGF 5天后，而不是暴露于BDNF后，NT-3和L1水平增加了3 ~ 4倍。低频刺激（0.1 Hz，5天）降低L1和trkC水平的情况下，神经生长因子。通过特定的冲动活动模式调节L1和trkC的表达可能会影响细胞相互作用，协调神经系统在关键发育阶段的结构和功能。

We studied the relationship between neuronal activity, neural cell adhesion molecules and neurotrophins in mouse dorsal root ganglion (DRG) neurons during development in vitro. The nervous system structure is modified by electrical activity in developing neural circuits, but the molecular mechanisms are not well under stood. Recognition molecules and neurotrophic factors regulate cell interactions during development. Therefore, the possible involvement of neural cell adhesion molecules (L1, NCAM and N-cadherin), and of neurotrophins (NGF, BDNF and NT-3) in activity-development was studied by delivering electrical pulses to mouse DRG neurons in cultures. After 5 days exposure to NGF, but not after exposure to BDNF, the NT-3 and L1 levels increased 3〜4 fold. Low frequency stimulation (0.1 Hz for 5 days) decreased L1 and trkC levels in the absence of NGF. Regulation of the expression of L1 and trkC by specific patterns of impulse activity may influence cell interactions coordinating structure and function of the nervous system at critical developmental stages.