Studies on equid herpesvirus 1 infection in mice.

用小鼠研究了马疱疹病毒1型(EHV-1)感染小鼠的保护性免疫机制、病毒血症发生率及对分娩的影响。取得了以下研究结果：1.结论：1.BALB/CA小鼠鼻腔感染EHV-1后7天内，CD4或CD8 T细胞亚群均可抑制EHV-1在肺内的生长。肺组织中病毒生长抑制与肺组织中肿瘤坏死因子活性无明显相关性，提示肿瘤坏死因子对EHV-1.3无抗病毒活性。EHV-L免疫小鼠再次感染时出现病毒血症，其发生率和持续时间与初次感染相似，但免疫小鼠耳部病毒的生长受到明显抑制。提示抗体作为一种抑制病毒在免疫小鼠肺内生长的机制。JN CB-17酸性小鼠肺内病毒生长受到某种非特异性机制的快速抑制，提示小鼠对EHV-1感染的保护机制不够深入。妊娠15~17天的BALB/CA小鼠鼻腔感染EHV-1，不仅可引起母鼠的临床症状，还可引起胎儿生长迟缓和死亡。然而，在任何接受测试的新生儿身上都无法证明该病毒。

Using mice, mechanism of protective immunity, incidence of viremia and effect on parturition were studied in equid herpesvirus 1 (EHV-1) infection. Following results were obtained.1. Growth of EHV-1 in lungs of BALB/cA mice was suppressed by either CD4 or CD8 T cell subset within 7days after intranasal infection.2. There was not significant correlation between suppression of virus growth in lung and TNF activity in lung, indicating no anti-viral activity of TNF against EHV-1.3. Viremia occurred in reinfection of EHV-l-immunized mice as similar incidence and duration to initial infection, although growth of the virus in lugs of immunized mice was markedly suppressed.4. As a mechanism of suppression of viral growth in lungs of immunized mice, antibody was indicated.5. Jn CB-17 acid mice, viral growth in lungs was quickly suppressed by a certain non-specific mechanism, indicating inadequate mice for analyzing protective mechanism against EHV-1 infection.6. Intranasal infection with EHV-1 in pregnant BALB/cA mice at 15 to 17 days of gestation resulted in not only clinical signs in dams, but also growth retardation and death of fetuses. However, the virus could not be demonstrated in any neonates tested.