Studies on equid herpesvirus 1 infection in mice.

小鼠马疱疹病毒 1 型感染的研究。

• 批准号: 09680833
• 负责人: IWAI Hiroshi
• 金额: $ 1.73万
• 依托单位: Rakuno Gakuen University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680833/
• 关键词: equid herpesvirus 1; mouse; model; 感染防御; 潜伏感染

Using mice, mechanism of protective immunity, incidence of viremia and effect on parturition were studied in equid herpesvirus 1 (EHV-1) infection. Following results were obtained.1. Growth of EHV-1 in lungs of BALB/cA mice was suppressed by either CD4 or CD8 T cell subset within 7days after intranasal infection.2. There was not significant correlation between suppression of virus growth in lung and TNF activity in lung, indicating no anti-viral activity of TNF against EHV-1.3. Viremia occurred in reinfection of EHV-l-immunized mice as similar incidence and duration to initial infection, although growth of the virus in lugs of immunized mice was markedly suppressed.4. As a mechanism of suppression of viral growth in lungs of immunized mice, antibody was indicated.5. Jn CB-17 acid mice, viral growth in lungs was quickly suppressed by a certain non-specific mechanism, indicating inadequate mice for analyzing protective mechanism against EHV-1 infection.6. Intranasal infection with EHV-1 in pregnant BALB/cA mice at 15 to 17 days of gestation resulted in not only clinical signs in dams, but also growth retardation and death of fetuses. However, the virus could not be demonstrated in any neonates tested.

本研究用小鼠研究了马疱疹病毒1型（EHV-1）感染后的保护性免疫机制、病毒血症发生率及对分娩的影响。主要研究结果如下：1.结论：1. BALB/cA小鼠经鼻感染EHV-1后7天内，CD 4或CD 8 T细胞亚群均能抑制EHV-1在肺内的生长.肺内病毒生长抑制与肺内TNF活性无明显相关性，表明TNF对EHV-1.3无抗病毒活性。EHV-1免疫小鼠再次感染后出现病毒血症，其发生率和持续时间与初次感染相似，但病毒在免疫小鼠耳中的生长受到明显抑制.表明抗体是抑制病毒在免疫小鼠肺中生长的一种机制.在CB-17酸性小鼠中，病毒在肺中的生长被某种非特异性机制迅速抑制，表明该小鼠不适合分析EHV-1感染的保护机制.在妊娠15 ~ 17天的BALB/cA小鼠中，EHV-1鼻内感染不仅导致母鼠的临床症状，而且导致生长迟缓和胎儿死亡。然而，在任何受试新生儿中均未发现该病毒。

项目成果

Iwai, H.et al.: "Effect of Equid Herpesvirus 1 Infection on Parturition of Mice" J.Equine Sci.9. 25-27 (1998)

Iwai, H.等人：“马疱疹病毒 1 感染对小鼠分娩的影响”J.Equine Sci.9。

Iwai,H et al.: "Effect of Equid Herpesvirus1 Infection on Parturition of Mice." J.Equine Sci.9・1. 25-27 (1988)

Iwai, H 等：“马疱疹病毒 1 感染对小鼠分娩的影响。”J.Equine Sci.9·1（1988）。

Iwai, H., Kodera, A., Sekiya, N., Nakamura, A., and Kirisawa, R.: "Effect of Equid Herpesvirus 1 Infection on Parturition of Mice" J.Equine Sci.9-1. 25-27 (1998)

Iwai, H.、Kodera, A.、Sekiya, N.、Nakamura, A. 和 Kirisawa, R.：“马疱疹病毒 1 感染对小鼠分娩的影响”J.Equine Sci.9-1。