Establishment of a new extraction method for phytochemicals using the wet stone-mill and non-destructive evaluation method for extract quality based on fluorescence excitation emission matrix

在原有湿式磨石机的基础上，成功地建立了一种新的萃取方法——同步磨矿萃取工艺（SMEP）。以乙醇70%为最佳提取溶剂，1环磨为最佳提取条件，对SMEP进行了优化。在最佳提取条件下，SMEP提取总多酚、总黄酮和抗氧化能力均显著高于所有比较提取方法，甚至大大简化了提取流程、缩短了提取时间的超声提取方法。此外，通过与癌症相关的人视黄醛脱氢酶3型（RALDH3）酶抑制的生化试验，证实了smep生产的提取物的实际用途。smep提取的提取物活性明显高于其他方法制备的提取物。因此，SMEP在生产高质量香料提取物和SMEP生产的香料提取物方面的实用性得到了证实。当前项目的第一和第三个目标的这些结果导致了一篇原创论文的发表（Bui et al., 2022），第69届JSFST会议的优秀海报奖和两次邀请演讲。

The new extraction method named the Simultaneous Milling and Extraction Process (SMEP) has been successfully established using the original wet stone-mill. SMEP was successfully optimized with ethanol 70% as the most suitable extraction solvent and 1 loop milling as the most efficient setting. SMEP at its optimal condition demonstrated significantly higher extraction efficiency in terms of total polyphenols, total flavonoids and antioxidant abilities compared to all comparative extraction methods, even the ultrasonic-based methods with greatly simplified protocol and in shorter time. In addition, the practical usage of SMEP-produced extracts was confirmed with the biochemical assay involving the cancer-related human retinaldehyde dehydrogenase type 3 (RALDH3) enzyme inhibition. SMEP-produced extracts were significantly more active than extracts prepared by other methods. Therefore, the practical usability of SMEP in producing spice extracts of high quality and of SMEP-produced extracts has been confirmed. These results of the first and third objectives in the current project led to publication of an original paper (Bui et al., 2022), an Excellent Poster Award in the 69th JSFST Conference and two invited lectures.