Establishment of a new extraction method for phytochemicals using the wet stone-mill and non-destructive evaluation method for extract quality based on fluorescence excitation emission matrix

利用原有的湿式石磨，成功地建立了一种新的提取方法，即同时研磨和提取工艺（SMEP）。成功地优化了SMEP，其中70%乙醇作为最合适的提取溶剂，1个循环研磨作为最有效的设置。在其最佳条件下，SMEP在总多酚，总黄酮和抗氧化能力方面表现出显着更高的提取效率，与所有比较提取方法相比，即使是基于超声波的方法，大大简化了方案，在更短的时间内。此外，SMEP生产的提取物的实际用途通过涉及癌症相关的人视网膜醛脱氢酶3型（RALDH 3）酶抑制的生物化学测定来确认。SMEP生产的提取物比其他方法制备的提取物活性更高。因此，SMEP在生产高质量的香料提取物和SMEP生产的提取物中的实际可用性已经被证实。当前项目的第一个和第三个目标的这些结果导致了一篇原创论文的发表（Bui等人，2022年），在第69届JSFST会议优秀海报奖和两个邀请讲座。

The new extraction method named the Simultaneous Milling and Extraction Process (SMEP) has been successfully established using the original wet stone-mill. SMEP was successfully optimized with ethanol 70% as the most suitable extraction solvent and 1 loop milling as the most efficient setting. SMEP at its optimal condition demonstrated significantly higher extraction efficiency in terms of total polyphenols, total flavonoids and antioxidant abilities compared to all comparative extraction methods, even the ultrasonic-based methods with greatly simplified protocol and in shorter time. In addition, the practical usage of SMEP-produced extracts was confirmed with the biochemical assay involving the cancer-related human retinaldehyde dehydrogenase type 3 (RALDH3) enzyme inhibition. SMEP-produced extracts were significantly more active than extracts prepared by other methods. Therefore, the practical usability of SMEP in producing spice extracts of high quality and of SMEP-produced extracts has been confirmed. These results of the first and third objectives in the current project led to publication of an original paper (Bui et al., 2022), an Excellent Poster Award in the 69th JSFST Conference and two invited lectures.