Establishment of a new extraction method for phytochemicals using the wet stone-mill and non-destructive evaluation method for extract quality based on fluorescence excitation emission matrix

在原有湿式磨石机的基础上，成功地建立了一种新的萃取方法--同时碾磨-萃取法。以70%乙醇为最适提取溶剂，1圈球磨为最优工艺条件，成功地优化了SMEP工艺。在最佳提取条件下，SMEP在总多酚、总黄酮和抗氧化能力方面的提取效率显著高于所有比较的提取方法，甚至比基于超声波的提取方法更简单，更短的时间。此外，SMEP产生的提取物的实际用途通过涉及癌症相关的人视黄醛脱氢酶3(RALDH3)酶抑制的生化检测得到证实。SMEP制备的提取物比其他方法制备的提取物具有更高的活性。因此，SMEP在生产高质量的香料提取物和SMEP生产的提取物方面的实用性得到了证实。本项目的第一个和第三个目标的这些成果导致发表了一篇原创论文(BUI等人，2022年)，在第69届JSFST会议上获得了一项优秀海报奖，并举办了两次特邀讲座。

The new extraction method named the Simultaneous Milling and Extraction Process (SMEP) has been successfully established using the original wet stone-mill. SMEP was successfully optimized with ethanol 70% as the most suitable extraction solvent and 1 loop milling as the most efficient setting. SMEP at its optimal condition demonstrated significantly higher extraction efficiency in terms of total polyphenols, total flavonoids and antioxidant abilities compared to all comparative extraction methods, even the ultrasonic-based methods with greatly simplified protocol and in shorter time. In addition, the practical usage of SMEP-produced extracts was confirmed with the biochemical assay involving the cancer-related human retinaldehyde dehydrogenase type 3 (RALDH3) enzyme inhibition. SMEP-produced extracts were significantly more active than extracts prepared by other methods. Therefore, the practical usability of SMEP in producing spice extracts of high quality and of SMEP-produced extracts has been confirmed. These results of the first and third objectives in the current project led to publication of an original paper (Bui et al., 2022), an Excellent Poster Award in the 69th JSFST Conference and two invited lectures.