Study of synergistic effects among different environmental mutagens in the stamen hairs of Tradescantia

不同环境诱变剂对紫露草雄蕊毛的协同效应研究

• 批准号: 08680565
• 负责人: ICHIKAWA Sadao
• 金额: $ 1.6万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680565/
• 关键词: Tradescantia; stamen-hair system; somatic mutation; synergism; antagonism; alkylating agent; promutagen; peroxidase; マレイン酸ヒドラジド

This study was carried out in 1996 to 1998, using the stamen-hair system of Tradescantia clone BNL 4430, in order to investigate (1) the mutagenicities of alkylating agents and their mutagenic synergisms with X rays, (2) the mutagenic synergisms among alkylating agents, (3) the mutagenicities of promutagens and their mutagenic interactions with X rays, and (4) the mutagenic interactions between promutagens and alkylating agents.(1) The mutagenicities of N-ethyl-N-nitrosourea (ENU), diethyl sulfate (DES) and 1 , 2-dibromoethane (EDB) were determined, adding to those determined earlier for ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), dimethyl sulfate (DMS) and N-methyl-N-nitrosourea (MNU). ENU, DES and EDB all acted synergistically with X rays, as EMS, MMS and DMS did. (2) MNU acted merely additively with EMS, differing from MMS and EMS which acted synergistically. (3) The mutagenicities of o-phenylenediamine (PDA), N-nitrosodimethylamine (DMN) and EDB (also a bitunctional alkylating agent) were determined, and PDA and DMN were found to act synergistically with X rays when treated after exposing to X rays but antagonistically when treated before exposing to X rays, similarly to maleic hydrazide (MH) studed earlier. However, PDA and DMN acted synergistically and additively with X rays, respectively, when X-rayed during PDA and DMN treatments, indicating different time periods needed for their activations by peroxidase. (4) MH and EMS were found to act always antagonistically.

本研究于1996年至1998年进行，利用紫露草克隆BNL 4430的雄蕊毛系统，旨在研究（1）烷化剂的致突变性及其与X射线的致突变协同作用，（2）烷化剂之间的致突变协同作用，（3）促诱变剂的致突变性及其与X射线的致突变相互作用。 (4) 促诱变剂和烷化剂之间的诱变相互作用。 (1) 确定了 N-乙基-N-亚硝基脲 (ENU)、硫酸二乙酯 (DES) 和 1, 2-二溴乙烷 (EDB) 的诱变性，添加到先前确定的甲磺酸乙酯 (EMS)、甲磺酸甲酯 (MMS)、二甲基磺酸盐的诱变性。 硫酸盐（DMS）和N-甲基-N-亚硝基脲（MNU）。 ENU、DES 和 EDB 都与 X 射线协同作用，就像 EMS、MMS 和 DMS 一样。 (2)MNU与EMS仅相加作用，不同于MMS和EMS的协同作用。 (3)测定了邻苯二胺(PDA)、N-亚硝基二甲胺(DMN)和EDB(也是双功能烷基化剂)的致突变性，发现PDA和DMN在X射线照射后处理时与X射线具有协同作用，但在X射线照射前处理时则具有拮抗作用，与马来酰肼(MH)类似。 之前学过。然而，在 PDA 和 DMN 处理过程中进行 X 射线照射时，PDA 和 DMN 分别与 X 射线协同作用和相加作用，表明过氧化物酶激活它们所需的时间不同。 (4)发现MH和EMS总是拮抗作用。

项目成果

Shima N.et al.: "Young inflorescence-bearing shoots with roots of Tradescantia clone BNL 4430 cultivated in nutrient solution circulating systems : an alternative to potted plants and cuttings for mutagenicity tests." Mutation Research. 395. 199-208 (1997

Shima N.等人：“在营养液循环系统中培养的带有紫露草克隆 BNL 4430 根的幼花序芽：用于诱变性测试的盆栽植物和插条的替代品。”

Xiao L.Z. and Ichikawa S.: "Dicentric chromosome bridges in root tips and micronuclei in pollen tetrads induced by X rays and maleic hydrazide in Tradescantia clone BNL 4430." Genes and Genetic Systems. 72. 187-195 (1997)

肖丽珍

Xiao L.Z. and Ichikawa S.: "Peroxidase activities in the floral tissues or Tradescantia clone BNL 4430 treated with maleic hydrazide alone,X rays alone,or in combinations" Gene and Genetic Systems. 71. 151-157 (1996)

肖丽珍

Xiao L.Z. and Ichikawa S.: "Antagonistic effects of ethyl methanesulfonate and maleic hydrazide in inducing somatic mutations in the stamen hairs of Tradescantia clone BNL 4430" Genes and Genetic Systems. 73. 287-292 (1998)

肖丽珍

Shima N.and Ichikawa S.: "Synergistic effects of N-ethyl-N-nitrosourea (an alkylating agent with a low Swain-Scott substrate constant) and X-rays in the stamen hairs of Tradescantia clone BNL 4430." Environmental and Molecular Mutagenesis. 29(in press). (

Shima N. 和 Ichikawa S.：“N-乙基-N-亚硝基脲（一种具有低 Swain-Scott 底物常数的烷化剂）和 X 射线对紫露草克隆 BNL 4430 雄蕊毛的协同作用。”