The establishment of total risk assessment method for water pollution included sediment.

近年来，由重金属、农药、除草剂、洗涤剂等引起的水生环境污染问题日益严重。此外，对沉积物中积聚的化学物质的毒性的主要规定也需要进行总风险评估。为了评估化学物质对沉积物的污染，我们建立了一种新的生物测定系统，使用秀丽隐杆线虫（c.e elegans）和细胞培养。认为利用RT-PCR分析mrna可以筛选环境污染及其胁迫条件。在本研究中，我们试图评估金属硫蛋白、细胞色素P450IA和血红素加氧酶mRNA水平对水生环境污染的影响。在细胞培养研究中，我们开发了一些沉淀物毒性的生物测定方法，如armarblue ^<TM>测定法和DNA radder和comet测定法。我们建立的这些生物标志物是环境化学物质毒性的良好指标。另一方面，卵黄原蛋白mRNA的诱导能力也可能提示其化学成分会对内分泌干扰起焦虑作用。这是通过调节免疫、代谢、形态发生、神经和生殖功能来完成的。研究结果表明，所建立的方法在实验室和野外工作中都是一种有效的预警系统，可用于评价沉积物的环境污染。

Recently, it becomes very serious problem that an aquatic environmental pollution caused by heavy metals, pesticides, herbicide and detergents. In addition, a major provision of the toxicity for there chemicals which accumulate within sediment also required for the total risk assessment. For assessing the sediment contamination with chemicals, we established a new bioassay system using Caenorhabditis elegans (C.elegans) and a cell culture. It is considered that the analysis of mRNAs using RT-PCR will enable us screen the environmental pollution and its stress conditions. In this study, we tried to evaluate mRNA levels of metallothionein, cytochrome P450IA and heme oxygenase for aquatic environmental pollution. In cell culture study, we developed some bioassaies for toxicity of sediment such as aramar Blue^<TM> assay and DNA radder and comet assay. These biomarkers which we established are very good indicator for the toxicity for environmental chemicals. On the other hand, the induction ability of vitellogenin mRNA may also suggest that its chemical will be anxious for the action of endocrine disruption. This is accomplished through regulation of irmmune, metabolic, morphogenic, neural and reproductive functions.The results of my study, in conclusion, the established method is a useful means as an alarm system both in the laboratory and field work for assessment of environmental pollution for sediment.