UV-induced apoptasis and photorepair

紫外线诱导的细胞凋亡和光修复

• 批准号: 08680566
• 负责人: MITANI Hiroshi
• 金额: $ 1.54万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680566/
• 关键词: Medaka; Apoptosis; UV; Photolyase; Cell death

UV radiation from three wavelengths, i.e.UVA (340-400 nm), UVB (290-320 nm) and UVC (200-290 nm), were shown to induce apoptosis in OCP13 cells by DNA ladder formation in cells detached from the plastic dish after irradiation. Morphological changes of the irradiated cells occurred in 4 hours after each wavelength UV.However, the following phenomena only occurred with UVB or UVC irradiation, but not with UVA irradiation. 1), UV-induced detachment of cells from substratum was inhibited with cycloheximide treatment. 2), the characteristic features of the morphological changes after UV irradiation, such as distorted nuclear shape and rearrangement of cells occurred. 3), the morphological changes described above could be evaded by photorepair. 4), the cells attached on both plastic dish and coverslip shows similar morphological changes after UV irradiation. 5), caspase 3, which is activated in apoptosis process in mammalian cells, was also highly activated after UV irradiation.These results indicate a different pathway between long (UVA) and short (UVB and UVC) wavelength UV-induced apoptosis. UVA-induced apoptosis would not be triggered through CPDs or requires caspase 3 activity as UVB and UVC-induced apoptosis.

来自三种波长的紫外线辐射，即UVA（340-400 nm）、UVB（290-320 nm）和UVC（200-290 nm），可通过照射后从塑料培养皿中分离的细胞中形成DNA梯状结构来诱导OCP 13细胞凋亡。各波长紫外线照射后4小时内，细胞均发生形态学变化，但以下现象仅在UVB或UVC照射下发生，在UVA照射下不发生。放线菌酮处理可抑制紫外线诱导的细胞脱离基质。（2）紫外线照射后细胞形态发生改变的特征，如细胞核变形、细胞重排等。（3）光修复可避免上述形态学变化。4），贴壁细胞与盖玻片细胞经紫外线照射后形态学变化相似。结果表明，长波紫外线（UVA）和短波紫外线（UVB和UVC）诱导的细胞凋亡途径不同，长波紫外线（UVA）和短波紫外线（UVC）诱导的细胞凋亡途径不同。UVA诱导的细胞凋亡不像UVB和UVC诱导的细胞凋亡那样通过CPD触发或需要caspase 3活性。

项目成果

Wang B.: "Apoptosis induction in cultured mouse embryonic mid brain cells." Radiation Research. 147. 304-308 (1997)

Wang B.：“培养的小鼠胚胎中脑细胞的凋亡诱导。”

Mitani,H.: "Induction of cyclobutane pyrimidine dimer photolyase in cultured fish cells by UVA and blue light." Photochem.Photobiol.64. 943-946 (1996)

Mitani, H.：“通过 UVA 和蓝光在培养鱼细胞中诱导环丁烷嘧啶二聚体光裂合酶。”

Wang B., Fujita, K., Watanabe, K., Mitani, H., Yamada, T., and Shima A.: "Apoptosis induction in cultured mouse embryonic mid brain cells." Radiation Research. 147. 304-308 (1997)

Wang B.、Fujita, K.、Watanabe, K.、Mitani, H.、Yamada, T. 和 Shima A.：“培养的小鼠胚胎中脑细胞中的细胞凋亡诱导。”

Funayama,T.: "Over expression of Medaka(Oryzias latipes)photolyase gene in Medaka cultured cells and early embryos." Photochem.Photobiol.63. 633-638 (1996)

Funayama,T.：“青鳉（Oryzias latipes）光解酶基因在青鳉培养细胞和早期胚胎中过度表达。”

三谷啓志: "“ストレスタンパク質"" ストレスとホルモン(比較内分泌学会編)学会出版センター, 26 (1997)

Keishi Mitani：“‘应激蛋白’”应激与激素（比较内分泌学会编辑）学会出版中心，26（1997）