Development of DNA repair assay for ionizing radiation damages.

开发电离辐射损伤的 DNA 修复测定。

• 批准号: 04680208
• 负责人: MITANI Hiroshi
• 金额: $ 1.34万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04680208/
• 关键词: Ionizing Radiation; Plasmid; DNA; Oxygen; γ線; 無細胞系

Ionizing radiation induce the formation of different and diverse types of DNA lesions. Oxygen free radicals produced by ionizing radiation generate single and doublestrand breaks as well as a variety of ring-saturated, ring-fragmented, and oxidized base derivatives. The repair mechanisms of such lesions are not well investigated. I tried to develop the cell free repair system to study the repair in vitro. Plasmids DNA are irradiated and mixed with cell extract and rejoining of DNA strand breaks, recovery of transcription and repair DNA synthesis. The repair of DNA irradiated with gamma ray was detected with this systems. And I am now try to develop this system for quantitative analysis. I also find the oxygen stress induce the some repair systems for UV induced lesions and we are now examining the effect of oxygen stress on DNA repair for ionizing radiation induced damages.

电离辐射诱导形成不同类型的DNA损伤。电离辐射产生的氧自由基产生单链和双链断裂以及各种环饱和、环断裂和氧化的碱基衍生物。此类病变的修复机制尚未得到很好的研究。我尝试建立无细胞修复系统，在体外研究修复。质粒DNA被照射并与细胞提取物混合，并重新连接DNA链断裂，恢复转录和修复DNA合成。用该系统检测了γ射线照射后DNA的修复。我现在正在尝试开发这个系统进行定量分析。我还发现氧应激诱导了一些紫外线损伤的修复系统，我们现在正在研究氧应激对电离辐射损伤的DNA修复的影响。

项目成果

Mitani, H., and Shima, A.: "Induction of cyclobutane pyrimidine dimer photolyase in cultured fish cells by fluorescent light and oxygen stress." Photochem. Photobil.(in press).

Mitani, H. 和 Shima, A.：“通过荧光和氧应激在培养鱼细胞中诱导环丁烷嘧啶二聚体光裂合酶。”

Funayama,T: "ultraviolct-induced DNA damage and its photorepain in taut fin cells of the Medaka,Oryzias latipes" Photochem.Photobiol.58. 380-385 (1993)

Funayama,T：“紫外线诱导的青鳉鱼鳍细胞中的 DNA 损伤及其光修复”Photochem.Photobiol.58。

Uchida, N., Mitani, H., and Shima, A.: "Multiple effects of fluorescent light on repair of ultraviolet-induced DNA lesions in cultured goldfish cells." Photochem. Photobiol.61. 79-83 (1994)

Uchida, N.、Mitani, H. 和 Shima, A.：“荧光灯对修复培养金鱼细胞中紫外线诱导的 DNA 损伤的多重影响。”

Kondo, M., Mitani, H., and Shima, A.: "The effect of growth-inhibiting treatments on photorepair in cultured fish cells." Photochem.Photobiol.60. 120-124 (1994)

Kondo, M.、Mitani, H. 和 Shima, A.：“生长抑制处理对培养鱼细胞光修复的影响。”

Kondo,M: "The effect of grouth-inhibiting treatments on photorepair in cultured fish cells." Photochem Photobiol. 60. 120-124 (1994)

Kondo，M：“生长抑制处理对培养鱼细胞光修复的影响。”