Immediate and delayd type-allergic reactivity of disinfection by-products in drinking water

饮用水中消毒副产物的立即型和延迟型过敏反应

• 批准号: 08680585
• 负责人: UENO Hitoshi
• 金额: $ 1.6万
• 依托单位: Setsunan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680585/
• 关键词: drinking water; disinfection by-product; adjuvant; immunotoxicity; degranulation; chlorine; ozone; allergy; B細胞幼若化反応; T細胞幼若化反応; 活性酸素

The purpose of this study is to clarify the effects of disinfectants and various disinfection by-products (DBPs) on immune response. In the previous study, aldehydes such as glyoxal and chloral hydrate were found to generate active oxygen species and show more inhibitory action to B-cell mitogenic response than T-cell mitogenic response. In the current year, organic disinfection by-products (DBPs) were investigated for effects on immediate allergic reaction using an index of chemical mediator release, that is the release of beta-hexosaminidase from rat basophilic leukemia RBL-2H3 cells. In the stimulated degranulation condition with the complex of avidin and biotinylated mouse anti-DNP IgE antibody, none of DBPs affected the release of beta-hexosaminidase. In the normal condition, however, dichloroacetaldehyde, 2,4-dichlorophenol and 2,4,6-trichloro-phenol showed weak promoting action on the release of beta-hexosaminidase. To elucidate adjuvant activity of chlorination by-products as a mixture on IgE antibody production, mice were administered intraperitoneally with DNP-OVA and/or chlorinated humic acid-XAD 4/8-concentrate. The anti-DNP IgE antibody production to DNP-OVA was enhanced by the co-administration of Freund's incomplete adjuvant as a positive control, but rather lowered by the co-administration of chlorinated humic acid concentrate. The B/T cell ratio in spleen cells was elevated by the co-administration of DNP-OVA and chlorinated humic acid concentrate, compared to the case of the administration of DNP-OVA only. These results indicate that chlorination by-products affect immune responses and chlorinated humic acid concentrate as their mixture may rather suppress immune reaction.

本研究的目的是阐明消毒剂和各种消毒副产物（DBP）对免疫反应的影响。在之前的研究中，发现乙二醛和水合氯醛等醛类物质会产生活性氧，并且对B细胞促有丝分裂反应的抑制作用强于对T细胞促有丝分裂反应的抑制作用。今年，使用化学介质释放指标（即大鼠嗜碱性白血病 RBL-2H3 细胞释放的 β-己糖胺酶）研究了有机消毒副产物 (DBP) 对即时过敏反应的影响。在用抗生物素蛋白和生物素化小鼠抗 DNP IgE 抗体复合物刺激脱粒条件下，没有任何 DBP 影响 β-己糖胺酶的释放。然而，在正常条件下，二氯乙醛、2,4-二氯苯酚和2,4,6-三氯苯酚对β-己糖胺酶的释放表现出较弱的促进作用。为了阐明氯化副产物作为混合物对 IgE 抗体产生的佐剂活性，给小鼠腹膜内施用 DNP-OVA 和/或氯化腐殖酸-XAD 4/8-浓缩物。作为阳性对照的弗氏不完全佐剂的共同施用增强了针对DNP-OVA的抗DNP IgE抗体的产生，但是通过氯化腐殖酸浓缩物的共同施用反而降低了抗DNP IgE抗体的产生。与仅给予DNP-OVA的情况相比，通过同时给予DNP-OVA和氯化腐植酸浓缩物，脾细胞中的B/T细胞比率升高。这些结果表明，氯化副产物影响免疫反应，而氯化腐植酸浓缩物，因为它们的混合物可能会抑制免疫反应。