Composite application of high purified and recombinant human bone morphogenetic protein to the biomaterials.

1. 重组人骨形态发生蛋白-2 （rhBMP-2）在肌肉部位、皮下部位、骨骼部位和联合皮瓣部位具有稳定的成骨作用，而纯化BMP （pBMP）可诱导丰富的骨基质，其成骨活性约为相同剂量BMP-2.2的10倍。将含有10μg rhBMP-2和I型胶原的冻干多孔羟基磷灰石盘（n=15）植入兔下颌骨颊侧骨缺损。植入21天后，骨小梁排列在孔壁上。在中央部分，观察到骨髓包括血管样组织。在多孔羟基磷灰石盘的部分外表面观察到新的骨小梁形成。将冻干钛植入物和10μg的rhBMP-2和I型胶原（n=6）放置于犬下颌骨。植入后12周，螺纹间出现骨形成。在BMP刺激的种植体表面显示高百分比的骨接触。在反向扭矩强度方面，添加pBMP的钛种植体在短时间内获得较高的结合强度。这些结果表明，多孔羟基磷灰石与BMP是有用的框架材料重建广泛的骨缺损。使用带BMP的钛种植体有可能缩短固定植入手术与基台连接手术之间的时间。将2 μg的rhBMP-2与不同量的重组人碱性生长因子（bFGF, 0、16、80、400ng, 2、10、50μg）混合后植入大鼠小腿肌肉。植入后3周，rhBMP-2在低剂量bFGF作用下增强成骨活性，在高剂量bFGF作用下减弱成骨活性。这些结果表明，与bFGF同时治疗对rhBMP-2的成骨活性有双期影响。

1. Recombinant human bone morphogenetic protein-2 (rhBMP-2) indicated stable osteoinduction at muscle sites, subcutaneous sites, skeletal sites, and combined flap sites, however, purified BMP (pBMP) induces rich bone matrix and indicated about tenfold of osteoinducing activity than same dose of rhBMP-2.2. The lyophilized porous hydroxyapatite disk with 10μg of rhBMP-2 and type I collagen (n=15) were implanted into the surgically created bone defect of the buccal side on the rabbit mandible. At 21 days after implantation, trabecular bone lined pore walls. In the central portion, the bone marrow including angioid tissue was observed. New trabecular bone formation was observed on portions of the external surface of the porous hydroxyapatite disk.The lyophilized titanium implants and 10μg of rhBMP-2 and type I collagen (n=6) were placed in dog mandibles. At 12 weeks after implantation, Bone formation was shown between the threads. High percentage of bone contact at the BMP stimulated implant surfaces was indicated. In the reverse torque strength, titanium implants with pBMP obtains high bond strength within a short time period.These results suggests that porous hydroxyapatite with BMP is useful as a framing materials for reconstruction of widespread bone defects. The use of the titanium implants with BMP has the possibility of shortening the time required between fixture implantation surgery and abutment connection surgery.3. Two μg of rhBMP-2 and different amounts of recombinant human basic growth factor (bFGF; 0, 16, 80, and 400ng, and 2, 10, and 50μg) were mixed and implanted into rat calf muscles. Three weeks after implantation, rhBMP-2 increased osteoinducing activity with low dose of bFGF and decreased with high dose bFGF. These results suggests that concomitant treatment with bFGF shows biphasic effects to the osteoinducing activity by rhBMP-2.