The mechanism for synthesis and secretion of apo A-IV in CaCo-2 cells

CaCo-2细胞中apo A-IV的合成和分泌机制

• 批准号: 09672353
• 负责人: SEISHIMA Mitsuru
• 金额: $ 1.86万
• 依托单位: Gifu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672353/
• 关键词: Apo A-IV; Fatty acids; CaCo-2 cell; CaCoー2cell

Human apo A-IV is an unique apolipoprotein which is synthesized only in enterocytes and is involved in lipid absorption. However, the mechanism for synthesis and secretion of apo A-IV has not been fully explored. In this study, the effect of two kinds of fatty acid, oleic (CィイD218:1ィエD2) and caprylic (CィイD28:0ィエD2) acids, on apo A-IV synthesis and sedretion was examined in CaCo-2 cells. CaCo-2 cells, which are cell line origenated from human colon cancer cells and show characteristics of intestinal epithelim, were cultured on the filter mambrane of Transwell for 6 hr at 37℃ with 3.0 mM of oleic or caprylic acid with a micellar form. Oleic acid increased triglyceride secretion by 4 times, whereas caprylic acid increased by 1.3 times, compared to control. Apo A-IV concentration in the medium was significantly decreased in oleic acid, compared to caprylic acid. In contrast, apo A-IV mRNA level in cells is significantly increased in oleic acid compared to caprylic acid. It is suggested that caprylic acid enhances the secretion of apo A-IV without the increase in synthesis and oleic acid increases the transcription rate of apo A-IV, at least in CaCo-2 cells. These different effects of fatty acids on apo A-IV depends on the different carbon number of fatty acids.

人载脂蛋白A-IV是一种独特的载脂蛋白，仅在肠上皮细胞中合成，并参与脂质吸收。然而，载脂蛋白A-IV的合成和分泌机制尚未完全探索。本研究观察了油酸（C_（218）：1 ~（218）D_2）和辛酸（C_（218）：0 ~（218）D_2）对CaCo-2细胞apoA-IV合成和分泌的影响。CaCo-2细胞是来源于人结肠癌细胞的细胞系，具有肠上皮细胞的特征。与对照组相比，油酸使甘油三酯分泌增加4倍，而辛酸增加1.3倍。与辛酸相比，油酸中培养基中的Apo A-IV浓度显著降低。相反，与辛酸相比，油酸中细胞中的apo A-IV mRNA水平显著增加。这表明，辛酸增强载脂蛋白A-IV的分泌，而不增加合成和油酸增加载脂蛋白A-IV的转录速率，至少在CaCo-2细胞。脂肪酸对apo A-IV的这些不同影响取决于脂肪酸的碳数不同。

项目成果

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