Study on analytical system for the etiology of autoimmune disease using two-dimensional nano LC/MS

二维纳米LC/MS自身免疫性疾病病因分析系统研究

• 批准号: 17390161
• 负责人: SEISHIMA Mitsuru
• 金额: $ 9.93万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390161/
• 关键词: clinical proteome; biomarker; clinical test; 質量分析; プロテオーム

We first examined about the pretreatment of serum for proteome analysis. However, the detected number of protein was rather reduced when the device for low-molecular protein was used. On the other hand, cerebrospinal fluid (Lupus 17:16-20, 2008) and brain tissue (Neunmi Lett 422:114-118, 2007) which are not abundant in albumin are suitable samples for the mass spectrometry analysis. Further, we could find the conformational change which is a post translational modification of nitrated IDO using this analytical system (J Immunol 176:372-379, 2006). We also showed that the determination of serum ceruloplasinin is useful for the differentiation of idiopathic thrombocytic purpura (ITP) (Clin Chim Acta 389:132-138, 2008). Moreover, we found out same new unidentified proteins as possible allergen from serum proteins in egg allergy patients (BBRC under submission).Useful biomarkers will be found when more effective pretreatment for serum is established.

我们首先研究了用于蛋白质组分析的血清预处理。然而，当使用用于低分子蛋白质的装置时，检测到的蛋白质的数量相当少。另一方面，白蛋白不丰富的脑脊液（Lupus 17：16-20，2008）和脑组织（Neunmi Lett 422：114-118，2007）是用于质谱分析的合适样品。此外，使用该分析系统，我们可以发现构象变化，其是硝化IDO的翻译后修饰（J Immunol 176：372-379，2006）。我们还表明，血清铜蓝蛋白的测定对于特发性血小板减少性紫癜（ITP）的鉴别是有用的（Clin Chim Acta 389：132-138，2008）。此外，我们从鸡蛋过敏患者的血清蛋白中发现了与可能的过敏原相同的新的未鉴定蛋白（BBRC正在提交中）。当建立更有效的血清预处理时，将发现有用的生物标志物。

项目成果

The measurement of serum ceruloplasmin is useful for Diagnostic differentiation of immune thrombocytopenic purpura

血清铜蓝蛋白测定有助于免疫性血小板减少性紫癜的诊断鉴别

• 发表时间: 2008
• 期刊: Clin Chim Acta 389
• 作者: Chousa;M
• 通讯作者: M

Proteomic analysis of autoantibodies in neuropsychiatric systemic lupus erythematosus patient with white matter hyperintensities on brain MRI

• DOI: 10.1177/0961203307085112
• 发表时间: 2008-01-01
• 期刊: LUPUS
• 影响因子: 2.6
• 作者: Kimura, A.;Sakurai, T.;Inuzuka, T.
• 通讯作者: Inuzuka, T.

Serum-soluble tumor factor receptor 2 (sTNF-R2) level determines clinical outcome in patients with aggressive non-Hodgkin's lymphoma.

血清可溶性肿瘤因子受体 2 (sTNF-R2) 水平决定侵袭性非霍奇金淋巴瘤患者的临床结果。

• 发表时间: 2006
• 期刊: European Journal of Haematology 77(3)
• 作者: Goto N;Tsurumi H;Takemura M;Hara T;Sawada M;Kasahara S;Kanemura N;Yamada T;Shimizu M;Takahashi T;Tomita M;Seishima M;Takami T;Moriwaki H.
• 通讯作者: Moriwaki H.

The measurement of serum ceruloplasmin is useful for diagnostic differentiation of immune thrombocytopenic purpura.

血清铜蓝蛋白的测量有助于诊断鉴别免疫性血小板减少性紫癜。

• 发表时间: 2008
• 期刊: Clin Chim Acta 389
• 作者: Chousa M;wt al.
• 通讯作者: wt al.

Proteomic analysis of autoantibodies in neuropsychiatric systemic lupus erythematosuspatient with white matter hyperintensiti

神经精神系统性红斑狼疮白质高信号患者自身抗体的蛋白质组学分析

• 发表时间: 2008
• 期刊: Lupus 17
• 作者: Kimura A;et. al.
• 通讯作者: et. al.