Epidemiologic study on methicillin-resistant S.aureus in the medical center

医疗中心耐甲氧西林金黄色葡萄球菌流行病学调查

• 批准号: 09672364
• 负责人: IGARI Jun
• 金额: $ 1.79万
• 依托单位: JUTENDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672364/
• 关键词: NICU; MRSA; PFGL; genotyping; phenotyping; フェノタイプ; SmaI; SstII; パルスフィールドゲル電気泳動法; genotyping; phenotyping

This study was conducted to know the distribution of methicillin-resistant S.aureus(MRSA) in the neonatal intensive care unit(NICU).We investigated MRSA carriers of medical staffs(7 medical doctors and 21 nurses) and 30 neonatal patients between May 1995 and January 1999. We studied the genotyping of isolated MRSA strains by pulsed-field gel electrophoresis(PFGL) using the macrorestriction enzyme, SmaI and the phenotyping by antibiotic resistant patterns as well.The antibiotic drups were vancomycin, oxacillin, ampicillin, cefazolin, cefmetazole, imipenem, gentamicin, minocycline, ofloxacin and clindamycin and the minimal inhibitory concentration(MIC) was measured by the broth microdilution method according to the guideline of NCCLS.The MRSA was isolated 21 strains from 17 nurses, 7 strains from 8 doctors and 49 strains from 30 patiants. In genotyping, all isolates except one isolate from a patient showed the same major type which was divides 6 subtypes.The phenotype had 4 types.The result of the genotyping study indicated that tile Same origin MRSA distributed in NICU.

为了解耐甲氧西林金黄色葡萄球菌（MRSA）在新生儿重症监护病房（NICU）的分布情况，于1995年5月~ 1999年1月对医护人员（7名医生，21名护士）和30例新生儿患者进行了MRSA带菌调查。本文应用脉冲场凝胶电泳（PFGL）技术，以SmaI为限制性内切酶，对临床分离的MRSA菌株进行了基因分型，并根据耐药谱进行了表型分型，所用抗生素为万古霉素、苯唑西林、氨苄西林、头孢唑啉、头孢美唑、亚胺培南、庆大霉素、米诺环素、氧氟沙星和克林霉素的最小抑菌浓度（MIC）结果17名护士、8名医生和30名病人分别分离出21株、7株和49株MRSA。基因分型结果显示，除1株患者分离株外，其余菌株均为同一主型，共分为6个亚型，表型有4种，基因分型结果表明，NICU中存在同源MRSA。

项目成果

田部陽子, 猪狩 淳: "特別養護老人ホーム入所者のMRSA保菌状況の経時的変化に関する検討" 環境感染. Vol.12,No3. 206-210 (1997)

Yoko Tabe，Jun Ikari：“特殊疗养院居民 MRSA 感染状况随时间变化的研究”《环境感染》，第 12 卷，第 3 期，206-210（1997 年）。