Precise Distinctions between Very Immature T-linegae and NK-lineage Neoplasms

重要著作（Blood 82：207，1993; Am J Hematol 45：150，1994;同上45：310，199 4 ;同上49：1995年6月）对来源于未成熟期的T细胞肿瘤（T-ALL/LBL）进行了表型和基因分析，强烈怀疑胸腺前期的CD 7 + CD 2+亚群，被认为是非常不成熟的T-谱系的一个亚群，似乎来源于NK-谱系的一个非常不成熟的阶段（Blood 89：4665，1997）。本研究的主要目的是对这一CD 7 + CD 2+亚群进行详细的研究。然而，与CD 7 + CD 5+组相比，该亚组的发病率如此之低，以至于在研究期间没有遇到任何此类新鲜病例。（在撰写本报告期间，发生了一起案件，目前正在调查中。）在10个表达TCR α_β的T-ALL/LBL细胞系、3个表达TCR γ_δ的T-ALL/LBL细胞系和一个成熟NK细胞系YT中研究了前T细胞受体α（preT细胞受体α）的表达分布。[1]在表达TCR α链后，表达足够量的前T α。[2]在3个表达TCR γ_δ的T-ALL/LBL细胞系中，由于TCR β基因的单克隆重排，前T α（preT α）均出现了异常表达，这两个结果都是较新的发现。

Important works (Blood 82 : 207,1993 ; Am J Hematol 45 : 150,1994 ; ibid 45 : 310,199 4 ; ibid 49 : 6.1995) have been done by our group regarding phenptypic and gene ana Lysis of T-cell neoplasms derived from immature stages, T-ALL/LBL.It was strongly suspected that CD7+CD2+ subset of pro-thymic stage, which had been regarded as a subset of very immature T-lineage, seemed to be derived from an very immature stage of NK-lineage (Blood 89 : 4665,1997). The detailed research of this CD7+CD2+ subgroup is the major goal of this research. However, the incidence of this subgroup has been so low compared with CD7+CD5+ group that any such fresh case has not been encountered during the research peripod. (During writing this report, one case has been experiences, and the investigation is now going on.)The distribution of the expression of preTalpha (pre T-cell receptor alpha) has been studied in 10 TCRalpha_beta expressing T-ALL/LBL cell lines, 3 TCRgamma_delta expressing T-ALL/LBL cell lines and a mature NK cell line, YT.[1] After expressing TCRalpha chain, enough amount of preTalpha is expressed.[2] In the 3 TCRgamma_delta expressing T-ALL/LBL cell lines, which have the monoclonal rearrangement of TCR beta gene, preTalpha are unexpectedly expressed.These two are quite new observations.