The functional role of photosynthesis-related genes in non-photosynthetic symbionts of corals

我们收集了珊瑚群并进行了DNA提取。为了鉴定与珊瑚相关的顶复门（Corallicolida目，也称为corallicolids），使用18 S rRNA区域的特异性引物进行PCR扩增和桑格测序。初步结果显示，来自日本和印度-太平洋其他地区的大多数珊瑚与相同的多面手珊瑚科物种有关。此外，利用16 S rRNA（细菌）、18 S rRNA（真核生物）和ITS 2 rRNA（共生藻科的鞭毛藻）引物，文库制备和MiSeq测序用于评估与珊瑚相关的微生物的多样性。我们根据已发表的管道使用生物信息学分析了数据。初步结果显示，珊瑚与共生藻科的枝藻属、杜氏藻属和共生藻属共生。此外，来自大西洋和印度洋-太平洋的珊瑚具有不同的共生藻科组成。在细菌方面，共生的内共生单胞菌属是珊瑚最常见的共生体。一些样品与弧菌属有关，它们是机会致病菌。到目前为止，来自印度太平洋不同地区的样本分析了不同的细菌组成。

We collected coral colonies and performed DNA extraction. In order to identify apicomplexans associated with corals (order Corallicolida, also called corallicolids), PCR amplification was conducted using specific primers for the 18S rRNA region and Sanger sequencing. Preliminary results indicated that most corals from Japan and other parts of the Indo-Pacific Ocean associated with the same generalist corallicolid species. Still, some corals samples were associated with a distinct corallicolid lineage.Additionally, library preparation and MiSeq sequecing was used to evaluate the diversity of microbes associated with corals, using 16S rRNA (bacteria), 18S rRNA (Eukaryotes), and ITS2 rRNA (diniflagellates of the family Symbiodiniaceae) primers. We analyzed the data with bioinformatics following published pipelines. Preliminary results showed that corals associated with Symbiodiniaceae of the genera Cladocopium, Durisdinium and Symbiodinium. Moreover, corals from the Atlantic and the Indo-Pacific oceans have a different Symbiodiniaceae composition. In relation to bacteria, the mutualistic genus Endozoicomonas was the most common symbiont of corals. Some samples were associated with Vibrio spp., which are opportunistic pathogens. Samples from different regions on the Indo-Pacific Ocean analyzed so far had a different bacterial composition.