Function and mechanism of the lysosomal polypeptide transporter ABCB9 (P09)

ABCB9形成同二聚体复合体，功能上可分为核心复合体和n端跨膜结构域tmd0，负责溶酶体定位并与LAMP-1/2相互作用。我们将用核磁共振波谱法确定分子结构。此外，将研究TMD0与其交互伙伴之间的接口。用细胞生物学方法研究ABCB9的溶酶体转运机制。肽结合和释放以及ATP水解将通过单分子技术和快速动力学进行分析。

ABCB9 forms a homodimeric complex, which can functionally be divided into the core complex and the N-terminal, transmembrane domainTMD0, responsible for lysosomal localization and interaction with LAMP-1/2. We will determine the molecular structure by NMR spectroscopy. Further, the interface between TMD0 and its interaction partners will be investigated. With cell biological methods the machinery for lysosomal trafficking of ABCB9 will be studied. Peptide binding and release as well as ATP hydrolysis will be analyzed by single molecule techniques and fast kinetics.