Differentiation and Proliferation of the Growth-plate Cartilage in the Transplantation of the Apophyseal Grwth-plate into the Full-thickness Defect of the Articular Cartilage

骨突生长板移植至关节软骨全层缺损中生长板软骨的分化与增殖

• 批准号: 09671499
• 负责人: TAKEDA Yoshitsugu
• 金额: $ 0.77万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671499/
• 关键词: articular cartilage; growth plate; repair; apoptosis; MHC; transplantation; 成長期関節軟骨; 関節軟骨修復; DNA電気泳動

Transplants of the apophyseal growth plate obtained from the ilium of Lewis rats at 4 weeks of age were inserted into the full-thickness defects in the patellofemoral articular surface of Fisher, LEC and LEA rats. Lewis rat and Fisher rat have the same Major Histocompatibility Antigen (MHC), and MHC is different between Lewis rat and LEC / LEA rats. LEC rat and LEA rat possesses the same MHC, but LEC rat lack the CD4_+ T-cell function congenitally. Femoral specimens including transplants were cut and fixed in formarin and paraffin embedded. The tissue sections were studied by in situ end-labeling procedure at one-week intervals up to 4 weeks in order to detect the apoptosis.In each recipient, no staining of cells with morphological characteristics of apoptosis was demonstrated in growth plate until the incorporation of the grafts to the defects. But apoptotic cells were detected in bone marrow under the graft at 1 and 2 weeks after transplantation. These findings were similar to that of autografts.The results of this study suggested that the immunological response of the recipients do not affct on the apoptosis that has been developed in the donor site by allograft of the apophyseal growth plate into the full-thickness defects in the patellofemoral articular surface.

将取自 4 周龄 Lewis 大鼠髂骨的骨突生长板移植物插入 Fisher、LEC 和 LEA 大鼠髌股关节面全层缺损处。 Lewis大鼠和Fisher大鼠具有相同的主要组织相容性抗原(MHC)，而Lewis大鼠和LEC/LEA大鼠之间的MHC不同。 LEC大鼠和LEA大鼠具有相同的MHC，但LEC大鼠先天缺乏CD4_+ T细胞功能。将包括移植物在内的股骨标本切割并固定在包埋的福尔马林和石蜡中。为了检测细胞凋亡，每隔一周至四周通过原位末端标记程序对组织切片进行研究。在每个受者中，在移植物并入缺损之前，生长板中没有显示出具有细胞凋亡形态学特征的细胞染色。但移植后1周和2周时，移植物下骨髓中检测到凋亡细胞。这些结果与自体移植物的结果相似。这项研究的结果表明，受体的免疫反应不会影响通过将骨突生长板同种异体移植到髌股关节面全层缺损中而在供体部位产生的细胞凋亡。

项目成果

武田芳嗣 他: "新鮮成長軟骨板移植による成長期関節軟骨修復" 移植(日本移植学会雑誌). 33. 192 (1998)

Yoshitsugu Takeda 等人：“通过移植新鲜生长的软骨板来修复生长的关节软骨”移植（日本移植学会杂志）33. 192（1998）。

武田芳嗣 他: "新鮮成長軟骨板移植による成長期関節軟骨修復" 移植(日本移植学会雑誌). 33. 192- (1998)

Yoshitsugu Takeda 等：“通过移植新鲜生长的软骨板进行生长关节软骨修复”移植（日本移植学会杂志）33. 192-（1998）。

Yoshitsugu Takeda, Takaaki Ikata, Hiroshi Mikami, Takashi Umehara, Takashi Nishioka, Kunisuke Himeno: "Transplantation of the Growth-plate Cartilage into the Full-thickness Defect of the Articular Cartilage" Japanese Journal of Transplantation. VOL.33. 19

Yoshitsugu Takeda、Takaaki Ikata、Hiroshi Mikami、Takashi Umehara、Takashi Nishioka、Kunisuke Himeno：“生长板软骨移植到关节软骨全层缺损中”日本移植杂志。

梅原隆司, 井形高明, 武田芳嗣, 西岡 孝, 加藤謙治, 斉藤慎一郎, 佐野壽昭: "腸骨成長軟骨移植による関節軟骨修復でのアポトーシスについて" 第16回日本骨・関節・軟部組織移植研究会講演抄録集. 46 (1997)

Takashi Umehara、Takaaki Igata、Yoshitsugu Takeda、Takashi Nishioka、Kenji Kato、Shinichiro Saito、Toshiaki Sano：“关于髂生长软骨移植的关节软骨修复中的细胞凋亡”第16届日本骨/关节/软组织移植研究会讲座摘要集。 46 (1997)