Differentiation and Proliferation of the Growth-plate Cartilage in the Transplantation of the Apophyseal Grwth-plate into the Full-thickness Defect of the Articular Cartilage

将Lewis大鼠4周龄髂骨骺骺生长板移植于Fisher、LEC和LEA大鼠髌股关节面全层缺损处。Lewis大鼠与Fisher大鼠具有相同的主要组织相容性抗原（MHC）， Lewis大鼠与LEC / LEA大鼠的MHC不同。LEC大鼠与LEA大鼠具有相同的MHC，但LEC大鼠先天缺乏CD4_+ t细胞功能。包括移植物在内的股骨标本切开，用福尔马林和石蜡包埋固定。采用原位末端标记法，每隔一周至四周观察组织切片，以检测细胞凋亡情况。在每个受体中，生长板上没有细胞凋亡形态学特征的染色，直到移植物结合到缺陷中。但在移植后1周和2周，在移植物下的骨髓中检测到凋亡细胞。这些结果与自体移植物相似。本研究结果表明，受体的免疫反应不影响同种异体骨突生长板移植到髌股关节表面全层缺损的供体部位发生的细胞凋亡。

Transplants of the apophyseal growth plate obtained from the ilium of Lewis rats at 4 weeks of age were inserted into the full-thickness defects in the patellofemoral articular surface of Fisher, LEC and LEA rats. Lewis rat and Fisher rat have the same Major Histocompatibility Antigen (MHC), and MHC is different between Lewis rat and LEC / LEA rats. LEC rat and LEA rat possesses the same MHC, but LEC rat lack the CD4_+ T-cell function congenitally. Femoral specimens including transplants were cut and fixed in formarin and paraffin embedded. The tissue sections were studied by in situ end-labeling procedure at one-week intervals up to 4 weeks in order to detect the apoptosis.In each recipient, no staining of cells with morphological characteristics of apoptosis was demonstrated in growth plate until the incorporation of the grafts to the defects. But apoptotic cells were detected in bone marrow under the graft at 1 and 2 weeks after transplantation. These findings were similar to that of autografts.The results of this study suggested that the immunological response of the recipients do not affct on the apoptosis that has been developed in the donor site by allograft of the apophyseal growth plate into the full-thickness defects in the patellofemoral articular surface.