仮骨延長法における骨形成促進のための低出力レーザー利用

我们改进了一个实验系统，观察了氩激光对体外培养的成骨细胞系MCSTSE 1细胞的作用，发现氩激光对细胞膜、线粒体和DNA合成有损伤作用。Ga-Al-As激光对DNA合成有一定的激活作用，He-Ne激光对碱性磷酸酶（ALP）活性有一定的促进作用。随着照射时间的缩短，细胞损伤程度逐渐减轻。当照射能量降低时，未照射和照射部分之间没有差异。结果。该实验系统仅限于检测低输出激光照射后的影响。为此，我们改进了多孔板实验系统，将Ga-Al-As激光和He-Ne激光用透镜聚焦，照射MC 3 T3-E1细胞。改变每种激光的照射条件并进行评价，随着Ga-Al-As激光输出功率的增加，细胞增殖和ALP活性下降。He-Ne激光照射成骨细胞后，细胞增殖和ALP活性随照射时间的延长而受到抑制，这并不能确定最佳的成骨细胞活化条件。

We previously improved an experimental system to evaluate the effects of laser to clonal osteoblastic MCSTSE1 cells in a cultured flask.The argon laser was founci to damage the cell membrane, rnitochondria, and DNA synthesis. DNA synthesis was slightly activateci by the Ga-Al-As laser, and the alkaline phosphatase (ALP) activity was slightly increased by the He-Ne laser.Next, the irradiation condition was changed in our system. The cell damage decreased when the irradiation time was shortened. When the irradiation energy was reduced, no difference between the non-irradiation ancl irradiated portion was identified. As a result. this experimental system was limited to detect the influence after low output laser irradiation. Therefore, we improved an experimental system in a multiwell plate.The Ga-Al-As laser ancl He-Ne laser were paralleled by a lens and irradiated MC3T3-E1 cells. The irradiation conditions of each laser were changed ancl assessedl, As the output of the Ga-Al-As laser increased, the cell proliferation ancl ALP activity decreased. With the He-Ne laser, the cell proliferation ancl ALP activity were suppressed as the exposure time increased.This stucly did not determine the best conditions for activation of osteoblasts.