仮骨延長法における骨形成促進のための低出力レーザー利用

利用低功率激光促进骨形成的骨痂延长法

• 批准号: 09671525
• 负责人: SHIRAI Hisaya
• 金额: $ 0.64万
• 依托单位: Ortopedic Surgery of Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671525/
• 关键词: MC3T3E1 cells; Argon laser; Ga-Al-As laser; He-Ne laser; WST; ALP; ^3H-thymidine; MTT; レーザー照射; He-Neレーザー; アルカリホスファターゼ; WST-1; He-Heレーザー; 核酸

We previously improved an experimental system to evaluate the effects of laser to clonal osteoblastic MCSTSE1 cells in a cultured flask.The argon laser was founci to damage the cell membrane, rnitochondria, and DNA synthesis. DNA synthesis was slightly activateci by the Ga-Al-As laser, and the alkaline phosphatase (ALP) activity was slightly increased by the He-Ne laser.Next, the irradiation condition was changed in our system. The cell damage decreased when the irradiation time was shortened. When the irradiation energy was reduced, no difference between the non-irradiation ancl irradiated portion was identified. As a result. this experimental system was limited to detect the influence after low output laser irradiation. Therefore, we improved an experimental system in a multiwell plate.The Ga-Al-As laser ancl He-Ne laser were paralleled by a lens and irradiated MC3T3-E1 cells. The irradiation conditions of each laser were changed ancl assessedl, As the output of the Ga-Al-As laser increased, the cell proliferation ancl ALP activity decreased. With the He-Ne laser, the cell proliferation ancl ALP activity were suppressed as the exposure time increased.This stucly did not determine the best conditions for activation of osteoblasts.

我们之前改进了一个实验系统来评估激光对培养瓶中克隆成骨细胞MCSTSE1的影响。氩激光的作用是破坏细胞膜、线粒体和DNA合成。Ga-Al-As激光能轻微激活DNA合成，He-Ne激光能轻微提高碱性磷酸酶（ALP）活性。然后，在我们的系统中改变辐照条件。随着辐照时间的缩短，细胞损伤程度降低。当辐照能量降低时，未辐照部分和辐照部分之间没有差异。因此。该实验系统仅限于检测低输出激光辐照后的影响。因此，我们改进了多孔板实验系统。Ga-Al-As激光和He-Ne激光通过透镜平行照射MC3T3-E1细胞。实验结果表明，随着Ga-Al-As激光输出量的增加，细胞增殖能力和ALP活性下降。在He-Ne激光照射下，随着照射时间的延长，细胞增殖和ALP活性均受到抑制。本研究没有确定成骨细胞活化的最佳条件。

项目成果

Hiroshi Kato, Takumi Yonezawa, Hisaya Shirai, Kouichi Sano, Muneaki Abe: "An Improved method for the Evaluation of the Effects of Laser Irradiation on Cultured Osteoblastic Cells" Bulletin of the Osaka Medical College. vol.44 no.1. 19-26 (1998)

Hiroshi Kato、Takumi Yonezawa、Hisaya Shirai、Kouichi Sano、Muneaki Abe：“一种评估激光照射对培养成骨细胞效果的改进方法”大阪医学院通报。

Hiroshi Kato: "An Improved method for the Evaluation of the Effects of Laser Irradiation on Cultured Osteolastic Cells" Bulletin of the Osaka Medical College. 44・1. 19-26 (1998)

加藤博：“激光照射对培养的成骨细胞的影响的改进方法”大阪医学院通报44・1（1998）。

加藤 洋: "An Improved Method for the Evaluation of the Effects of Laser Irradiation on Cultured Osteoblastic Cells" BULLETIN OF THE OSAKA MEDICAL COLLEGE. 44・1. 19-26 (1998)

加藤博：“激光照射对培养成骨细胞的影响的改进方法”大阪医科大学通报44・1（1998）。