仮骨延長法における骨形成促進のための低出力レーザー利用

我们之前改进了一种实验系统，以评估激光对培养瓶中克隆的成骨细胞MCSTSE1细胞的影响。Ga-Al-As激光对DNA合成有轻微的激活作用，He-Ne激光对碱性磷酸酶(ALP)活性有轻微的促进作用。照射时间越短，细胞损伤越小。当辐照能量降低时，未辐照部分与辐照部分没有差别。结果。该实验系统仅限于检测低输出激光照射后的影响。为此，我们改进了多孔板上的实验系统，用透镜平行的Ga-Al-As激光器和He-Ne激光器照射MC3T3-E1细胞。改变各激光的照射条件，随着Ga-Al-As激光输出功率的增加，细胞增殖和碱性磷酸酶活力下降。在He-Ne激光照射下，随着照射时间的延长，细胞增殖和ALP活性均受到抑制，但这并不能确定成骨细胞激活的最佳条件。

We previously improved an experimental system to evaluate the effects of laser to clonal osteoblastic MCSTSE1 cells in a cultured flask.The argon laser was founci to damage the cell membrane, rnitochondria, and DNA synthesis. DNA synthesis was slightly activateci by the Ga-Al-As laser, and the alkaline phosphatase (ALP) activity was slightly increased by the He-Ne laser.Next, the irradiation condition was changed in our system. The cell damage decreased when the irradiation time was shortened. When the irradiation energy was reduced, no difference between the non-irradiation ancl irradiated portion was identified. As a result. this experimental system was limited to detect the influence after low output laser irradiation. Therefore, we improved an experimental system in a multiwell plate.The Ga-Al-As laser ancl He-Ne laser were paralleled by a lens and irradiated MC3T3-E1 cells. The irradiation conditions of each laser were changed ancl assessedl, As the output of the Ga-Al-As laser increased, the cell proliferation ancl ALP activity decreased. With the He-Ne laser, the cell proliferation ancl ALP activity were suppressed as the exposure time increased.This stucly did not determine the best conditions for activation of osteoblasts.