ARGON LASER FOR TIME-AND FREQUENCY-DOMAIN FLUORESCENCE

需要资金购买锁模氩离子激光器，一台高速(10 GHz)红敏微通道板(MCP)光电倍增管(PMT)，以及 近红外(NIR)染料激光器。荧光光谱(CFS)。CFS是一个 多用户设施，提供访问最先进的时间(TD)和 频域(FD)荧光仪器和分析程序 用于生物化学和生物物理学的研究。在启动阶段， CFS及其用户和合作者主要依赖于FD 拉科维奇博士实验室里的仪器。由于食品安全中心 仪器尚未完成，现有单一的NdYAG泵浦激光器 就足够了。这种情况现在已经改变了。食物安全中心现时有三个 时间分辨荧光仪器.GHz频域 时间相关单光子计数仪器(TCSPC，TD) 以及一种新开发的荧光寿命成像设备(FLIM)。 Flim方法允许生成二维图像，其中 对比度反映了局部荧光衰减时间。它不是 用一台泵操作这些仪器是可能的还是可行的 激光。 CFS仪表室和光学桌已经准备好了 Ar离子激光器，将与现有的染料激光器和 请求使用近红外染料激光器。将使用Ar泵浦的染料激光器 主要用于FD和FILM测量，其中强度和指向 稳定性是实验成功的关键。现有的NdYAG 激光将作为TCSPC的激光泵浦，它对 激光功率漂移。高速MCP光电倍增管和近红外染料激光器(苯乙烯 9M，790-900 nm)将用于支持研究中的用户和合作者 依赖于时间的光子迁移。CFS科学小组由以下人员组成 马里兰大学的核心用户和合作者， 来自其他大学的合作者和中心的外部用户。这个 Ar离子激光器将用于30个NIH支持的项目，9个NSF支持的项目 项目，外加其他约9个得到国家同行审查支持的项目。 这些项目涉及广泛的主题，包括对钙离子的研究。 结合蛋白、膜、血红蛋白、距离分布和 ATPase。最后，还有几个新的项目和/或合作者 时间分辨光子迁移、离子分布的研究 聚电解质，以及使用薄膜对心肌细胞中的钙进行成像。

Funds are requested for a mode-locked Argon Ion laser, a high speed (10 GHz) red-sensitive microchannel plate (MCP) photomultiplier tube (PMT), and near-IR (NIR) dye laser. Fluorescence Spectroscopy (CFS). The CFS is a multi-user facility which provides access to state-of-the-art time (TD) and frequency-domain (FD) fluorescence instrumentation and analysis programs for research in biochemistry and biophysics. During the start-up phase of the CFS its users and collaborators relied primarily on the FD instrumentation in Dr. Lakowicz's laboratory. Since the CFS instrumentation was not yet completed, the single existing NdYAG pump laser was adequate. This situation has now changed. The CFS now has three instruments for time-resolved fluorescence; a GHz frequency-domain instrument, a time-correlated single photon counting instrument (TCSPC, TD) and a newly developed apparatus for fluorescence lifetime imaging (FLIM). The FLIM method allows the generation of two-dimensional images in which the contrast reflects the local fluorescence decay time. It is not possible or practical to operate these instruments with a single pump laser. The CFS instrument room and its optical table is already prepared for an Argon Ion laser, which will be used with existing dye lasers and with the requested NIR dye laser. The Argon-pumped dye lasers will be used primarily for FD and FLIM measurements, where intensity and pointing stability are critical for successful experiments. The existing NdYAG laser will be used as the laser pump for TCSPC, which is less sensitive to drifts in laser power. The high speed MCP PMT and NIR dye laser (Styryl 9M, 790-900 nm) will be used to support users and collaborators in studies of time-dependent photon migration. The CFS scientific group consists of both core users and collaborators from the University of Maryland, collaborators from other universities, and external users of the CFS. The Argon Ion laser will be used for 30 NIH supported projects, 9 NSF supported projects, plus about 9 other projects with national peer-reviewed support. The projects involve widely ranging topics, including studies of Ca2+- binding proteins, membranes, hemoglobin, distance distributions, and ATPases. And finally, there are several new projects and/or collaborators on time-resolved photon migration, ion distribution around polyelectrolytes, and the use of FLIM to image calcium in cardiac cells.